The roots of Taraxacum kok-saghyz L.E. Rodin are rich in the storage carbohydrate inulin, which is known as a probiotic compound in human health. In this study, the relative expression of the inulin biosynthesis pathway genes (1-SST, 1-FFT, and 1-FEHI) was investigated in the roots, leaves, and cell suspension cultures. Also, the effect of 1-naphthaleneacetic acid (NAA) and methyl-jasmonate (MeJA) and the combination of both (MeJA/NAA) on T. kok-saghyz cell suspension culture is studied for the first time. The results showed a higher relative expression of the inulin biosynthesis genes in roots compared to leaves at both rosette and flowering stages. In addition, the relative expression of 1-SST and 1-FFT genes in the cell suspension was significantly reduced, while the 1-FEHI gene was upregulated compared to the roots. Regarding the results, low concentrations of NAA and MeJA were factors that strongly affected the expression of the 1-FFT gene, while the 1-SST gene showed high expression in low concentration NAA and its combination with MeJA. However, the relative expression of the 1-FEHI gene was significantly reduced in all treatments compared to the control. HPLC analysis results showed that the suspension culture treated with a low concentration of NAA had a higher amount of inulin than the control culture, which could be resulted due to the increased expression of 1-SST and 1-FFT genes. Results show that the low concentration NAA elicitation is a useful method for the enhancement of inulin production in cell suspension culture of T. kok-saghyz.

蒲公英的根含丰富的碳水化合物糖粉，这是人类健康中的益生菌化合物。在这项研究中，研究了菊粉生物合成途径基因（1-SST，1-FFT和1-FEHI）在根，叶和细胞悬浮培养物中的相对表达。此外，首次研究了1-萘乙酸（NAA）和茉莉酸甲酯（MeJA）以及两者的组合（MeJA / NAA）对T. kok-sa​​ghyz细胞悬浮培养的影响。结果表明，与玫瑰花结和开花期的叶片相比，菊粉生物合成基因在根中的相对表达更高。另外1-SST的相对表达与根系相比，细胞悬浮液中的1-FFT基因显着减少，而1-FEHI基因被上调。关于结果，低浓度的NAA和MeJA是强烈影响1-FFT基因表达的因素，而1-SST基因在低浓度的NAA及其与MeJA的组合中显示高表达。然而，与对照相比，在所有处理中1-FEHI基因的相对表达均显着降低。HPLC分析结果表明，用低浓度的NAA处理的悬浮培养物中的菊粉比对照培养物中的菊粉含量高，这可能是由于1-SST的表达增加所致和1- FFT基因。结果表明，低浓度的NAA诱导是提高T. kok-sa​​ghyz细胞悬浮培养物中菊粉产量的有用方法。