The present work was aimed to screen the antibacterial, anticancer and mosquito-larvicidal potentials of the titanium dioxide mediated nanoparticles (TiO₂NPs) from the Pleurotus sajor caju fruiting bodies. The synthesized TiO₂NPs were characterized using UV–Vis spectrum, FTIR, XRD, FESEM, EDX, HRTEM, Zeta potential and Particle size analyses. The XRD pattern show pure crystalline nature of the TiO₂NPs and spherical shape morphology was noted. The average size of nanoparticles was 85 nm and Zeta potential analysis, resulted the surface charge of the nanomaterial was − 8.05 mV. The larvicidal activity of TiO₂NPs treated IVth instar larvae of Aedes aegypti and Culex quinquefasciatus showed better LC₅₀ and LC₉₀ values (14.56, 26.56 and 12.76, 24.38 mg/l). The broad spectrum of antibacterial activity was observed both gram-positive and gram-negative bacteria and maximum growth inhibition zone was recorded in Klebsiella pneumoniae (8.0 ± 0.3 mm) followed by other tested organisms. The anticancer activity [bone cancer (MG-63) cell line] of TiO₂NPs expressed best IC₅₀ value as 61.34 µg/ml, which conferred the cytotoxic effects of TiO₂NPs on the proliferation of MG-63 cell line. Based on the findings of present study the biosynthesized TiO₂NPs from P. sajor caju served as an alternative and eco-friendly nano medicine in the near future.

目前的工作旨在筛选来自侧耳侧菇子实体的二氧化钛介导的纳米颗粒（TiO ₂ NPs）的抗菌，抗癌和灭蚊潜力。使用紫外可见光谱，FTIR，XRD，FESEM，EDX，HRTEM，Zeta电位和粒度分析对合成的TiO ₂ NPs进行了表征。XRD图谱显示了TiO ₂ NP的纯晶体性质，并且注意到球形形态。纳米粒子的平均尺寸为85 nm，并通过Zeta电位分析，得出纳米材料的表面电荷为-8.05 mV。二氧化钛的杀虫活性₂的纳米粒子治疗IVth龄幼虫埃及伊蚊和库克斯库蚊显示出更好的LC ₅₀和LC ₉₀值（14.56、26.56和12.76、24.38 mg / l）。在革兰氏阳性和革兰氏阴性细菌中均观察到了广泛的抗菌活性，在肺炎克雷伯菌（8.0±0.3 mm）中，随后是其他经过测试的生物，均记录到最大的生长抑制区。TiO ₂ NPs的抗癌活性[骨癌（MG-63）细胞系]的最佳IC ₅₀值为61.34 µg / ml，这赋予了TiO ₂ NPs对MG-63细胞系增殖的细胞毒性作用。根据目前的研究结果，从P. sajor caju生物合成的TiO ₂ NPs 在不久的将来用作替代和生态友好的纳米药物。