Molise region (Central-Southern Italy) is one of the Italian richest areas of truffles and contributes significantly to the national production of the precious Tuber magnatum. Nevertheless, Molise truffle has received little scientific attention. Accordingly, in the present study, two T. magnatum populations collected in two different sites of Molise region were characterised from a morphological, genetic and microbiological point of view. A considerable variability between and within the two analysed groups emerged, suggesting an interesting heterogeneity of Molise white truffle populations. Ascocarps of the two groups significantly differed in size and maturation degree, although no linear correlation between weight and maturity was found. Genetic investigations focused on the Sequence-Characterised Amplified Region SCAR A21-inf. Three haplotypes, randomly distributed within the two truffle groups regardless of their collection sites, were detected. The 16S rRNA gene amplicon high-throughput sequencing provided an overview of the composition of the ascocarp-associated bacterial communities. A predominance of α-Proteobacteria was observed, with Bradyrhizobium among the main genera. However, some truffles showed unusual microbial profiles, with Pedobacter, Polaromonas and other bacterial genera as dominant taxa.

中文翻译：

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意大利中南部有限地理区域白松露块茎的异质性

莫利塞地区（意大利中南部）是意大利松露最丰富的地区之一，对全国生产珍贵的大块茎做出了重大贡献。然而，莫利塞松露几乎没有受到科学的关注。因此，在本研究中，两个T。万能的从形态学、遗传和微生物学的角度对在莫利塞地区两个不同地点收集的种群进行了表征。两个分析组之间和内部出现了相当大的差异，表明莫利塞白松露种群存在有趣的异质性。两组的子囊果在大小和成熟度方面存在显着差异，但未发现重量与成熟度之间存在线性相关性。基因研究集中在序列特征的扩增区域 SCAR A21-inf。检测到三种单倍型，随机分布在两个松露组中，无论其采集地点如何。16S rRNA 基因扩增子高通量测序概述了与子囊果相关的细菌群落的组成。α-的优势观察到变形菌，主要属​​为慢生根瘤菌。然而，一些松露显示出不寻常的微生物特征，以Pedobacter、Polaromonas和其他细菌属为主要分类群。