Integrated otolith chemistry and muscle tissue stable isotope analyses were performed to allocate juvenile Diplodus puntazzo and Diplodus vulgaris to nurseries in the Adriatic Sea. Laser Ablation Inductively Coupled Plasma Mass Spectrometry (LA-ICP-MS) was used to quantify the concentrations of chemical elements in the otoliths. Fish muscle samples were analysed for δ¹³C and δ¹⁵N. In general, Ba/Ca and Sr/Ca ratios and isotopes varied between sites and species. Values of δ¹³C and δ¹⁵N were significantly different between species and sites. Multivariate analysis detected a significant difference in the element signature between species while there was no evidence for a significant interaction for sites. A clear pattern across the four groups of interest, D. puntazzo_Estuary > D. vulgaris_Estuary > D. puntazzo_Coastal > D. vulgaris_Coastal, following decreases in δ¹³C, and increases in δ¹⁵N were found. It seems that these species are feeding on the same local food web within more productive estuarine site while at costal site, feeding segregation among investigated species is evident. Both species were re-allocated correctly to the estuarine waters based on the otolith chemistry and stable isotopes information and higher value of δ¹⁵N. Combining otolith chemistry with tissue isotope ratios of juvenile fish provided complementary information on nursery habitat use at different spatial scales and elucidated ecological and environmental linkages.

中文翻译：

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耳石指纹图谱和组织稳定的同位素信息使幼鱼可以分配到不同的育苗区

进行了整合耳石化学和肌肉组织稳定同位素分析，以将幼龄双翅目和寻常双翅目分配给亚得里亚海的苗圃。激光烧蚀电感耦合等离子体质谱法（LA-ICP-MS）用于量化耳石中化学元素的浓度。鱼肌肉分析样品的δ ¹³ C和δ ¹⁵ N.在一般情况下，的Ba / Ca和Sr / Ca比值和同位素站点和物种之间变化。的δ值¹³ C和δ ¹⁵物种和地点之间的氮显着不同。多变量分析发现物种之间的元素特征存在显着差异，而没有证据表明位点之间存在显着的相互作用。在四个组感兴趣的图案清晰，D. puntazzo _Estuary> D.寻常_Estuary> D. puntazzo _Coastal> D.寻常_Coastal，在δ以下降低¹³ ℃，和在δ增加¹⁵发现了N。看来这些物种在河口地区生产力更高的地方以相同的食物网为食，而在沿海地区，被调查物种之间的饲料隔离是明显的。这两个物种被重新分配给正确基于所述耳石化学和稳定同位素δ的信息和更高的值河口水域¹⁵ N.耳石化学结合与幼鱼组织同位素比率设置在幼儿园栖息地使用在不同的空间尺度的补充信息和阐明了生态与环境的联系。