当前位置: X-MOL 学术Eur. J. Plant Pathol. › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Monocycle components of fig rust comparing in vivo and ex vivo methodology
European Journal of Plant Pathology ( IF 1.7 ) Pub Date : 2021-05-03 , DOI: 10.1007/s10658-021-02284-x
Eliane Aparecida Rogovski Czaja , Walmes Marques Zeviani , Maristela Dalla Pria , Louise Larissa May De Mio

The fig rust (Cerotelium fici) is the upmost important disease in fig but there are few studies on the effect of temperature and wetness period on the pathogen and on the disease. To improve the epidemiological knowledge of this pathosystem the germination of urediniospores and monocycle components were studied: incubation period (IP), latent period (LP) and number of pustules/leaf area, comparing ex vivo and in vivo methodology. For germination, temperatures tested ranged from 15 to 32 °C, with wetness periods of 6, 12 and 24 h, under continuous dark. The comparative study of the monocycle of the disease tested inoculations on leaf discs (ex vivo) and on plants (in vivo) with temperature ranging from 15 to 32 °C and 12 h/12 h light/dark, assessing the number of pustules of the first cycle of infection. Uredinospores germination had the optimal temperature from 25.8 to 28.3 °C increasing with wetness period. The shortest IP and LP were at temperature of 25.6 and 25.1 °C in the leaf discs methodology and 23.7 to 24.9 °C in the leaves on plants methodology. The estimation of the optimal temperature for number of pustules were similar between methods, ex vivo was 23.9 °C and in vivo was 22.9 °C. Similar results were obtained in both methodologies tested. We provide epidemiology bases of fig rust monocycle and we validated ex vivo methodology for this pathosystem; the methodology used is easier, cheaper and less time consuming when comparing to the use of in vivo essays.



中文翻译:

无花果铁锈的单环成分在体内和离体方法的比较

无花果锈病(Cerotelium FICI)是无花果中最重要的疾病,但关于温度和湿度时间对病原体和疾病的影响的研究很少。为了提高对这种病理系统的流行病学知识,研究了脲原虫子孢子和单环化合物的萌发:潜伏期(IP),潜伏期(LP)和脓疱/叶面积的数量,比较了离体和体内方法。对于发芽,在连续黑暗条件下,测试温度范围为15至32°C,湿润时间为6、12和24 h。对该疾病单周期疾病的比较研究测试了叶盘(离体)和植物(离体)在15至32°C和12 h / 12 h光照/黑暗温度下的接种量,评估了该病的脓疱数量第一个感染周期。乌头孢子萌发的最佳温度为25.8至28。3°C随着湿度的增加而增加。叶盘法中最短的IP和LP温度分别为25.6和25.1°C,而植物叶中最短的IP和LP为23.7至24.9°C。方法之间的脓疱最佳温度估计值相似,离体为23.9°C,体内为22.9°C。在两种测试方法中均获得了相似的结果。我们提供了无花果锈病单环的流行病学基础,并验证了该病理系统的离体方法学;与使用体内论文相比,所使用的方法更容易,更便宜且耗时更少。方法之间的脓疱最佳温度估计值相似,离体为23.9°C,体内为22.9°C。在两种测试方法中均获得了相似的结果。我们提供了无花果锈病单环的流行病学基础,并验证了该病理系统的离体方法。与使用体内论文相比,所使用的方法更容易,更便宜且耗时更少。方法之间的脓疱最佳温度估计值相似,离体为23.9°C,体内为22.9°C。在两种测试方法中均获得了相似的结果。我们提供了无花果锈病单环的流行病学基础,并验证了该病理系统的离体方法。与使用体内论文相比,所使用的方法更容易,更便宜且耗时更少。

更新日期:2021-05-04
down
wechat
bug