Abstract

The present study compared the effects of two commonly-used dispersants, bovine serum albumin (BSA) and polyethylene glycol (PEG), on the biodistribution and toxicity of oxidized super-growth single-wall carbon nanotubes (oxSG) injected intravenously into mice over 3 months. About 1–2% of the injected dose (ID) of oxSG dispersed in BSA (oxSG-BSA) was present in the lungs at all time points. By contrast, about 15% of the ID of oxSG dispersed in PEG (oxSG-PEG) was present in the lungs at 1 day (D1), with accumulation decreasing to about 5% of the ID at 90 days (D90). About 70–80% of the IDs of both oxSG-BSA and oxSG-PEG were present in the liver at D1; by D90, about 15% of the IDs were cleared slowly (oxSG-BSA) or rapidly (oxSG-PEG). In the spleen, about 7% of the IDs of both oxSG-BSA and oxSG-PEG were present at all time points. The toxicities of oxSG-BSA and oxSG-PEG were comparable: no obvious signs of inflammation were observed on histological assessments of the lungs, liver, and spleen and on measurements of cytokine activity in blood plasma and tissue lysates. Concentrations of aspartate transaminase slightly increased at some time points in blood plasma, suggesting that oxSG-BSA and oxSG-PEG were slightly hepatoxic. Taken together, these results indicated that the dispersants had limited effect on the biodistribution and toxicity of oxSGs.

摘要

本研究比较了两种常用的分散剂牛血清白蛋白 (BSA) 和聚乙二醇 (PEG) 对静脉注射超过 3 岁小鼠的氧化超生长单壁碳纳米管 (oxSG) 的生物分布和毒性的影响。个月。大约 1-2% 的注射剂量 (ID) 的 oxSG 分散在 BSA (oxSG-BSA) 中，在所有时间点都存在于肺部。相比之下，约 15% 的 oxSG 分散在 PEG (oxSG-PEG) 中的 ID 在第 1 天 (D1) 时存在于肺中，在 90 天 (D90) 时积累量下降到 ID 的约 5%。oxSG-BSA 和 oxSG-PEG 约 70-80% 的 ID 在 D1 时存在于肝脏中；到 D90，大约 15% 的 ID 被缓慢清除 (oxSG-BSA) 或快速清除 (oxSG-PEG)。在脾脏中，约 7% 的 oxSG-BSA 和 oxSG-PEG 的 ID 在所有时间点都存在。oxSG-BSA 和 oxSG-PEG 的毒性相当：在肺、肝和脾的组织学评估以及血浆和组织裂解物中细胞因子活性的测量中，没有观察到明显的炎症迹象。血浆中天冬氨酸转氨酶的浓度在某些时间点略有增加，表明 oxSG-BSA 和 oxSG-PEG 具有轻微的肝毒性。总之，这些结果表明分散剂对 oxSGs 的生物分布和毒性的影响有限。表明 oxSG-BSA 和 oxSG-PEG 具有轻微的肝毒性。总之，这些结果表明分散剂对 oxSGs 的生物分布和毒性的影响有限。表明 oxSG-BSA 和 oxSG-PEG 具有轻微的肝毒性。总之，这些结果表明分散剂对 oxSGs 的生物分布和毒性的影响有限。