ABSTRACT

Biofilm formation, as adapting strategies, is the result of stressful conditions that Salmonella faces in hostile environments like surface water. We evaluated river water effect on Salmonella biofilm formation ability in terms of physical, morphological characteristics and chemical composition. A new morphotype SPAM (soft, pink and mucoid) was detected in Oranienburg strains S-76 and S-347 (environmental and clinical isolate). Oranienburg serotypes showed very marked behavior in adherence, pellicle liquid-air and resistance, being Oranienburg S-76 the strongest biofilm producer. All strains when exposed to river water presented an overlapping mucoid layer in the morphotype and increased their motility except Oranienburg S-347. The most motile was Typhimurium (control) and the least Infantis S-304 (clinical isolate). Mannose, glucose, galactose and ribose were the main biofilm sugar components; type and concentration of sugar suggest a morphotype/serotype dependent pattern. Strong morphotypes expressed in this study may be an effective protective strategy for Salmonella in hostile environments.

摘要

作为适应策略的生物膜形成是沙门氏菌在地表水等恶劣环境中面临的压力条件的结果。我们评估了河水对沙门氏菌的影响生物膜在物理、形态特征和化学成分方面的形成能力。在 Oranienburg 菌株 S-76 和 S-347（环境和临床分离物）中检测到一种新的形态型 SPAM（软、粉红色和粘液状）。Oranienburg 血清型在粘附、表膜液-气和阻力方面表现出非常显着的行为，是 Oranienburg S-76 最强的生物膜生产者。当暴露于河水时，所有菌株在形态型中都呈现出重叠的粘液层，并增加了它们的运动性，除了 Oranienburg S-347。最能运动的是鼠伤寒（对照）和最少的 Infantis S-304（临床分离物）。甘露糖、葡萄糖、半乳糖和核糖是生物膜的主要糖成分；糖的类型和浓度表明存在形态型/血清型依赖性模式。恶劣环境中的沙门氏菌。