Ascochyta fabae is a fungal pathogen responsible for marked yield losses in spring and winter faba beans worldwide. The aim of this genome-wide association study (GWAS) using 188 diverse winter faba bean inbred lines was to exploit earlier Ascochyta blight resistance studies and to identify new resistance loci. Phenotyping after artificial inoculation under controlled conditions revealed significant variation for all eight scored disease traits. This GWAS was based on 1829 AFLP marker and 229 SNP marker, including 17 so-called ‘guide’ SNP markers. The latter were identified by map fragment alignments between the consensus smap of Webb et al., (2016, Plant Biotechnology Journal, 14, 177–185) and three earlier published Ascochyta blight resistance studies. A total of 12 markers were found significantly associated with six traits, explaining 5.6% to 21.7% of the phenotypic variance. One ‘guide’ SNP on chromosome III co-localizes with the known resistance QTL Af1 on chromosome III. Probably nine new resistance trait-associated marker loci were identified which will improve resistance breeding on winter faba beans and support a broader inclusion of the crop into rotations.

中文翻译：

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通过全基因组关联分析蚕豆（Vicia faba）对真菌Ascochyta fabae的抗性遗传研究

Ascochyta fabae是一种真菌病原体，导致全球春季和冬季蚕豆产量显着下降。这项使用 188 个不同冬蚕豆自交系的全基因组关联研究 (GWAS) 的目的是利用早期的子叶枯病抗性研究并确定新的抗性基因座。在受控条件下人工接种后的表型分析显示，所有八个评分的疾病特征都有显着差异。该 GWAS 基于 1829 个 AFLP 标记和 229 个 SNP 标记，包括 17 个所谓的“引导”SNP 标记。后者是通过 Webb 等人 (2016, Plant Biotechnology Journal, 14, 177–185) 的共识 smap 和三个较早发表的Ascochyta之间的图谱片段比对鉴定的抗性研究。共发现 12 个标记与 6 个性状显着相关，解释了 5.6% 至 21.7% 的表型变异。染色体 III 上的一个“引导”SNP 与染色体 III 上已知的抗性 QTL Af1共定位。可能确定了九个新的抗性性状相关标记基因座，它们将改善冬蚕豆的抗性育种，并支持将作物更广泛地纳入轮作。