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Ultramorphological study of immature stages and male genitalia of forensically significant flesh fly Sarcophaga dux thomson, 1868 (Diptera: Sarchophagidae)
Journal of King Saud University-Science ( IF 3.7 ) Pub Date : 2021-05-04 , DOI: 10.1016/j.jksus.2021.101460
Rohit Kumar , Samy Sayed , Madhu Bala , Sayed-Ashraf Elarrnaouty , Jana Ivanič Porhajašová , Zuhair M. Mohammedsaleh , Abdullah F. Shater , Bassam M. Al-ahmadi

Objective

Sarcophaga dux Thomson is a well-known flesh fly species of medicinal and forensic importance and remain active throughout the year. Female of this fly lays first instars directly onto the carcass or on wounds of living host as it causes myiasis. Use of S. dux in forensic investigations is limited because identification of immature stages as well as adults by conventional means is very challenging.

Methods

To overcome this limitation, the adults of S. dux were collected and reared under laboratory conditions. Immature stages were analyzed by using scanning electron microscopy and male genitalic characters were analyzed by light microscopy. Adults were identified on the basis of male genitalic characters whereas, immature stages like larva and pupa were identified on the basis of various taxonomic characters i.e. cephaloskeleton, anterior and posterior spiracles, appearance of spiracular cavity, papillae on the caudal segments and shape/arrangements of spines.

Results and Conclusion

Through present study an effort was made for accurate identification of immatures and adults of this flesh fly species of medical and forensic importance. Illustrations presented here will help in forensic investigations in cases involving this flesh fly species.



中文翻译:

具有法医意义的肉蝇Sarcophaga dux thomson未成熟阶段和雄性生殖器的超形态学研究,1868年(双翅目:Sarchophagidae)

客观的

Sarcophaga dux Thomson是一种具有医学和法医学重要性的著名果蝇,并且全年都活跃。该蝇的雌虫将幼虫直接放在onto体上或活着宿主的伤口上,因为它会引起肌病。由于通过常规手段鉴定未成熟阶段以及成年个体非常具有挑战性,因此在法医调查中使用S. dux受到限制。

方法

为了克服这一限制,收集了杜氏链球菌的成虫并在实验室条件下饲养。通过扫描电子显微镜分析未成熟阶段,并通过光学显微镜分析男性生殖器特征。成年人是根据男性生殖器特征来鉴定的,而幼虫和等未成熟阶段则是根据各种分类特征来鉴定的,例如头骨骼,前,后气孔,鼻腔的出现,尾节上的乳头状和尾部的形状/排列。刺。

结果与结论

通过目前的研究,已经做出了努力,以准确地识别这种具有医学和法医学重要性的蝇类的未成熟和成年。此处提供的插图将有助于对涉及这种蝇蝇的案件进行法医调查。

更新日期:2021-05-14
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