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Chitosan nanoparticles as a promising candidate for liver injury induced by 2-nitropropane: Implications of P53, iNOS, VEGF, PCNA, and CD68 pathways
Science Progress ( IF 1.906 ) Pub Date : 2021-05-04 , DOI: 10.1177/00368504211011839
Sameerah Shaheen, Maha M Arafah, Aliah R Alshanwani, Laila Mohammed Fadda, Ahlam M Alhusaini, Hanaa M Ali, Iman H Hasan, Hanan Hagar, Fatima MB Alharbi, Alaa AlHarthii

The current article was designed to assess the role of chitosan nanoparticles (CNPs) in the management of hepatic injury induced by the hepatocarcinogen 2-nitropropane (2-NP). Rats were divided into three groups. The first group served as a control, the second group was injected with 2-NP, while the third group was treated with CNPs 1 h before 2-NP injection every other day for 4 weeks. The 2-NP injection upregulated serum AST and ALT activities, as well as hepatic TNF- α, IL-6, and MDA levels and the expression of vascular endothelial growth factor (VEGF) and caspase-3, whereas GSH contents and SOD activity were decreased. Immunohistochemistry investigations revealed that the hepatic protein expression of collagen I, inducible nitric oxide synthetase, proliferating cell nuclear antigen, cluster of differentiation, and p53 were upregulated. hematoxylin and eosin (H&E) and Masson’s trichrome stains supported the previous parameters, and CNPs ameliorated most of the previous biochemical parameters. CNPs achieved promising results in the limitation of 2-NP hepatotoxicity.



中文翻译:

壳聚糖纳米颗粒作为2-硝基丙烷诱发的肝损伤的有前途的候选者:P53,iNOS,VEGF,PCNA和CD68途径的意义

本文旨在评估壳聚糖纳米颗粒(CNP)在管理由肝致癌物2-硝基丙烷(2-NP)引起的肝损伤中的作用。将大鼠分为三组。第一组作为对照,第二组注射2-NP,而第三组每隔一天注射2-NP之前1小时用CNP治疗4周。2-NP注射上调了血清AST和ALT活性,以及​​肝TNF-α,IL-6和MDA水平以及血管内皮生长因子(VEGF)和caspase-3的表达,而GSH含量和SOD活性却升高了。减少了。免疫组织化学研究表明,胶原蛋白I,诱导型一氧化氮合成酶,增殖细胞核抗原,分化簇和p53的肝蛋白表达均被上调。苏木和曙红(H&E)以及Masson的三色染料支持以前的参数,而CNP改善了大多数以前的生化参数。CNPs在限制2-NP肝毒性方面取得了令人鼓舞的结果。

更新日期:2021-05-04
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