Ethylenediaminetetraacetic acid (EDTA) is one of the most popular chelating agents. This study was conducted to investigate the effect of EDTA on the growth and health physiology of beluga (38.47 ± 0.58 g) before and after heavy metal stress (HMS). Five experimental diets, including control, 0.5EDTA, 1EDTA, 1.5EDTA and 2EDTA, were formulated to feed fish for eight weeks. The results showed that fish was fed with dietary 1.5EDTA (108.37 g) had a higher weight gain as compared with control (p < 0.05). Blood performance in the control group after HMS decreased but not in fish fed supplemented diets with EDTA. EDTA reduced uric acid and creatinine levels in beluga. Fish fed dietary 1.5EDTA had a higher value of blood performance (55.17), glucose (50.3 mg/dl), superoxide dismutase (SOD U/ml) (75.8) and glutathione peroxidase (234.0 U/ml) after HMS as compared with the control. Additionally, lower values of uric acid (3.05 mg/dl), creatinine (0.27 mg/dl) and AST (61.47 U/ml) after HMS in the 1.5EDTA group were observed (p < 0.05). Correlation analysis revealed a strong positive correlation between EDTA level and protein efficiency, lipid efficiency, and blood performance and a strong negative correlation with some parameters after stress, including SOD, glucose, uric acid, creatinine and aspartate transaminase (p < 0.01). In conclusion, feeding fish with the 1.5EDTA diet improved growth and detoxified heavy metals.

中文翻译：

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EDTA 对暴露在污染压力下的白鲸 (Huso huso) 上的重金属进行解毒：生长性能、免疫血液学、血液生化和抗氧化活性

乙二胺四乙酸 (EDTA) 是最常用的螯合剂之一。本研究旨在研究 EDTA 对重金属应激 (HMS) 前后白鲸 (38.47 ± 0.58 g) 生长和健康生理的影响。配制五种实验饮食，包括对照、0.5EDTA、1EDTA、1.5EDTA 和 2EDTA，用于喂鱼八周。结果表明，饲喂日粮 1.5EDTA（108.37 g）的鱼与对照组相比体重增加更高（p < 0.05)。HMS 后对照组的血液性能下降，但喂食添加了 EDTA 的饲料的鱼没有下降。EDTA 降低了白鲸的尿酸和肌酐水平。与 HMS 相比，饲喂 1.5EDTA 的鱼的血液性能 (55.17)、葡萄糖 (50.3 mg/dl)、超氧化物歧化酶 (SOD U/ml) (75.8) 和谷胱甘肽过氧化物酶 (234.0 U/ml) 值更高控制。此外，在 1.5EDTA 组中观察到 HMS 后尿酸 (3.05 mg/dl)、肌酐 (0.27 mg/dl) 和 AST (61.47 U/ml) 的较低值 ( p < 0.05)。相关性分析显示，EDTA 水平与蛋白质效率、脂质效率和血液性能之间呈强正相关，与应激后某些参数呈强负相关，包括 SOD、葡萄糖、尿酸、肌酐和天冬氨酸转氨酶 ( p  < 0.01)。总之，用 1.5EDTA 饮食喂养鱼可以改善生长并清除重金属。