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Fast and pervasive transcriptomic resilience and acclimation of extremely heat-tolerant coral holobionts from the northern Red Sea [Ecology]
Proceedings of the National Academy of Sciences of the United States of America ( IF 9.412 ) Pub Date : 2021-05-11 , DOI: 10.1073/pnas.2023298118
Romain Savary, Daniel J. Barshis, Christian R. Voolstra, Anny Cárdenas, Nicolas R. Evensen, Guilhem Banc-Prandi, Maoz Fine, Anders Meibom

Corals from the northern Red Sea and Gulf of Aqaba exhibit extreme thermal tolerance. To examine the underlying gene expression dynamics, we exposed Stylophora pistillata from the Gulf of Aqaba to short-term (hours) and long-term (weeks) heat stress with peak seawater temperatures ranging from their maximum monthly mean of 27 °C (baseline) to 29.5 °C, 32 °C, and 34.5 °C. Corals were sampled at the end of the heat stress as well as after a recovery period at baseline temperature. Changes in coral host and symbiotic algal gene expression were determined via RNA-sequencing (RNA-Seq). Shifts in coral microbiome composition were detected by complementary DNA (cDNA)-based 16S ribosomal RNA (rRNA) gene sequencing. In all experiments up to 32 °C, RNA-Seq revealed fast and pervasive changes in gene expression, primarily in the coral host, followed by a return to baseline gene expression for the majority of coral (>94%) and algal (>71%) genes during recovery. At 34.5 °C, large differences in gene expression were observed with minimal recovery, high coral mortality, and a microbiome dominated by opportunistic bacteria (including Vibrio species), indicating that a lethal temperature threshold had been crossed. Our results show that the S. pistillata holobiont can mount a rapid and pervasive gene expression response contingent on the amplitude and duration of the thermal stress. We propose that the transcriptomic resilience and transcriptomic acclimation observed are key to the extraordinary thermal tolerance of this holobiont and, by inference, of other northern Red Sea coral holobionts, up to seawater temperatures of at least 32 °C, that is, 5 °C above their current maximum monthly mean.



中文翻译:

快速而广泛的转录组回弹力和来自北红海的极耐热的珊瑚整体生物的适应性[生态学]

来自北部红海和亚喀巴湾的珊瑚具有极高的耐热性。为了检查潜在的基因表达动态,我们暴露了Stylophora pistillata从亚喀巴湾到短期(几小时)和长期(几周)的热应激,海水峰值温度从最高每月平均温度27°C(基线)到29.5°C,32°C和34.5° C。在热应激结束时以及在基线温度下恢复期之后对珊瑚进行采样。通过RNA测序(RNA-Seq)确定珊瑚宿主和共生藻类基因表达的变化。珊瑚微生物组组成的变化是通过基于互补DNA(cDNA)的16S核糖体RNA(rRNA)基因测序来检测的。在所有高达32°C的实验中,RNA-Seq揭示了基因表达的快速而普遍的变化,主要是在珊瑚宿主中,随后大部分珊瑚(> 94%)和藻类(> 71)的基因表达均恢复了基线。 %)基因在恢复期间。在34.5°C下,弧菌),表明已经超过了致死温度阈值。我们的结果表明,S. PISTILLATA holobiont可以安装上的热应力的幅度和持续时间的快速和普遍的基因表达响应队伍。我们认为,观察到的转录组适应性和转录组适应性是该类钩虫以及据推断推断,其他北红海珊瑚类钩虫具有非凡的热耐受性的关键,直到海水温度至少为32°C(即5°C)高于其当前的最高每月平均值。

更新日期:2021-05-03
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