Pancreatic cancer is one of the most common malignancies worldwide. This study is aimed at searching the possible genetic mutations and the value of novel gene mutation in the DNA damage-inducible transcript 4 (DDIT4) and signaling pathway in pancreatic cancer. Polymerase chain reaction (PCR) was performed to amplify the DNA sequences of DDIT4 from patients with pancreatic ductal adenocarcinoma. In addition, we used IHC to detect the expression level of DDIT4 in patients with pancreatic cancer in different types of gene mutation. Double-labeled immunofluorescence was employed to explore the expression levels of DDIT4/LC3 and their potential correlation. Our work indicated the two novel stable gene mutations in DDIT4 mRNA 3-untranslated region (m.990 U>A and m.1246 C>U). Thirteen samples were found to have mutation in the DDIT4 3-untranslated regions (UTR). To further verify the influence of gene mutation on protein expression, we performed immunohistochemistry on different gene mutation types, and we found a correlation between DDIT4 expression and gene mutation, which is accompanied by nuclear staining deepening. In order to further discuss the clinical value of DDIT4 gene mutation, immunofluorescence suggested that the expression of DDIT4 colocated with LC3; thus, we speculated that DDIT4 mutation may be involved in autophagy in pancreatic cancer cell. In this study, we found mutation in the 3-UTR region of DDIT4, which may be associated with DDIT4 expression and tumor autophagy in pancreatic cancer tissues.

中文翻译：

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DDIT4胰腺癌的新型突变

胰腺癌是全球最常见的恶性肿瘤之一。这项研究的目的是寻找可能的遗传突变和胰腺癌的DNA损伤诱导转录本4（DDIT4）和信号通路中的新基因突变的价值。进行聚合酶链反应（PCR）以扩增胰腺导管腺癌患者DDIT4的DNA序列。此外，我们使用IHC检测了不同类型基因突变的胰腺癌患者中DDIT4的表达水平。双标记免疫荧光被用来探讨DDIT4 / LC3的表达水平及其潜在的相关性。我们的工作表明DDIT4 mRNA 3中的两个新的稳定基因突变-非翻译区域（m.990 U> A和m.1246 C> U）。发现十三样品必须在DDIT4 3突变-非翻译区（UTR）。为了进一步验证基因突变对蛋白质表达的影响，我们对不同的基因突变类型进行了免疫组织化学分析，发现DDIT4表达与基因突变之间存在相关性，并伴随着核染色的加深。为了进一步讨论DDIT4基因突变的临床价值，免疫荧光提示DDIT4的表达与LC3共存。因此，我们推测DDIT4突变可能与胰腺癌细胞的自噬有关。在这项研究中，我们在3发现突变-DDIT4的UTR区，可能与胰腺癌组织中的DDIT4表达和肿瘤自噬有关。