A microcosm study was established using three arable soils differing in both physicochemical properties and AOB or AOA dominance of ammonia oxidization. Nitrification and N₂O emissions were quantified under 10 Pa C₂H₂, 1-octyne, or 3,4-dimethylpyrazole phosphate (DMPP), and the community composition and abundance of AOA and AOB were assessed. Amendment of soil with DMPP had a comparable effect to 1-octyne on AOB activities, composition, and N₂O emissions across soils. By blocking AOB activity, both DMPP and 1-octyne supported higher rates of AOA growth in both the acid and alkaline soils. Approximately, 50% of ammonia-oxidizing activity in the acid soil was attributed to AOB activity by phylotypes affiliated with Nitrosomonas communis. When AOB were blocked by DMPP or 1-octyne across all soils, recovery of AOA communities was associated with the increased abundance of Nitrososphaera viennesis.

使用三种在物理化学性质和氨氧化的AOB或AOA优势方面均不同的耕地建立了微观研究。硝化和N _2个O排放物在10帕Ç定量₂ ħ ₂，1-辛炔或3,4-二甲基吡唑磷酸盐（DMPP）和AOA和AOB的群落组成和数量进行了评估。DMPP改良土壤对土壤中AOB活性，组成和N ₂ O排放的影响与1-辛炔相当。通过阻止AOB活性，DMPP和1-辛炔在酸性和碱性土壤中均支持较高的AOA生长速率。酸性土壤中约有50％的氨氧化活性归因于与亚硝化单胞菌。当DMPP或1-辛炔在所有土壤中阻断AOB时，AOA群落的恢复与维也纳硝化球菌的丰度增加有关。