Abstract

Biofilm formation by Staphylococcus lugdunensis involves formation of an extracellular matrix; however, the identity of the constituents responsible for the structure of biofilms fabricated by different clinical strains is largely unclear. Here, biofilms produced by 24 clinical isolates of S. lugdunensis were characterized. The optimal medium for S. lugdunensis was selected, and the biofilm-forming capacity was assessed. Extracelullar polymeric substances (EPS) contributing to biofilm robustness were determined by evaluating the susceptibility of biofilms to EPS-degrading agents using field emission scanning electron microscopy and confocal laser scanning microscopy. Biofilm formation by the clinical isolates of S. lugdunensis was augmented by glucose supplementation. Further, extracellular DNA (eDNA), proteins, and polysaccharides were present in the 24 clinical isolates. Proteins and polysaccharides were the most common components within the S. lugdunensis biofilms, whereas the eDNA content was marginal in biofilm formation. Therefore, proteins and polysaccharides within biofilms may be used as the primary targets for developing eradication strategies to prevent S. lugdunensis biofilm formation.

摘要

卢氏葡萄球菌的生物膜形成涉及细胞外基质的形成。然而，对于由不同临床菌株制造的生物膜结构负责的成分的身份还不清楚。在这里，表征了由24种lugdunensis的临床分离株产生的生物膜。选择了卢格链霉菌的最佳培养基，并评估了其生物膜形成能力。通过使用场发射扫描电子显微镜和共聚焦激光扫描显微镜评估生物膜对EPS降解剂的敏感性来确定有助于生物膜坚固性的胞外聚合物质（EPS）。芦丁链球菌临床分离株的生物膜形成通过补充葡萄糖来增加。此外，在24种临床分离物中还存在细胞外DNA（eDNA），蛋白质和多糖。蛋白质和多糖是lugdunensis生物膜中最常见的成分，而eDNA含量在生物膜形成中却很少。因此，生物膜中的蛋白质和多糖可用作开发根除策略以预防卢格氏链球菌生物膜形成的主要目标。