Abstract

The present study was undertaken to elucidate the direct ALA effects on mice TM4 Sertoli cells proliferation in vitro. Our results showed that TM4 cells viability was significantly stimulated by ALA (p < 0.05). The 50 μM ALA increased the concentration of total antioxidant capacity, induced the mitochondrial membrane hyperpolarized, and markedly decreased the number of apoptosis cells (p < 0.05). ALA also up-regulated G2/Mitotic-specific cyclin-B1 gene and apoptosis suppressive gene Bcl2 expression (p < 0.05). In conclusion, those results indicated that ALA could increase TM4 Sertoli cells antioxidant capacity, induced the mitochondrial membrane hyperpolarized, inhibited cells apoptosis and stimulated TM4 Sertoli cells proliferation in vitro.

摘要

本研究旨在阐明 ALA 对小鼠 TM4 Sertoli 细胞体外增殖的直接影响。我们的结果表明，ALA 显着刺激了 TM4 细胞的活力 ( p <  0.05)。50 μM ALA 增加了总抗氧化能力的浓度，诱导线粒体膜超极化，并显着减少凋亡细胞的数量 ( p <  0.05)。ALA 还上调 G2/有丝分裂特异性细胞周期蛋白 B1 基因和凋亡抑制基因Bcl2的表达（p < 0.05）。总之，这些结果表明 ALA 可以增加 TM4 Sertoli 细胞的抗氧化能力，诱导线粒体膜超极化，抑制细胞凋亡并刺激 TM4 Sertoli 细胞体外增殖。