C-type lectin-like domain family 16 member A (CLEC16A) is associated with autoimmune disorders, including multiple sclerosis (MS), but its functional relevance is not completely understood. CLEC16A is expressed in several immune cells, where it affects autophagic processes and receptor expression. Recently, we reported that the risk genotype of an MS-associated single nucleotide polymorphism in CLEC16A intron 19 is associated with higher expression of CLEC16A in CD4⁺ T cells. Here, we show that CLEC16A expression is induced in CD4⁺ T cells upon T cell activation. By the use of imaging flow cytometry and confocal microscopy, we demonstrate that CLEC16A is located in Rab4a-positive recycling endosomes in Jurkat TAg T cells. CLEC16A knock-down in Jurkat cells resulted in lower cell surface expression of the T cell receptor, however, this did not have a major impact on T cell activation response in vitro in Jurkat nor in human, primary CD4⁺ T cells.

中文翻译：

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探索多发性硬化易感基因 CLEC16A 在 T 细胞中的作用

C 型凝集素样域家族 16 成员 A ( CLEC16A ) 与自身免疫性疾病有关，包括多发性硬化症 (MS)，但其功能相关性尚未完全了解。CLEC16A在几种免疫细胞中表达，它影响自噬过程和受体表达。最近，我们报道了CLEC16A内含子 19 中MS 相关单核苷酸多态性的风险基因型与CD4 ⁺ T 细胞中CLEC16A 的更高表达相关。在这里，我们表明CLEC16A表达在 CD4 ⁺T 细胞激活后的 T 细胞。通过使用成像流式细胞术和共聚焦显微镜，我们证明 CLEC16A 位于 Jurkat TAg T 细胞的 Rab4a 阳性回收内体中。Jurkat 细胞中的CLEC16A敲低导致 T 细胞受体的细胞表面表达降低，然而，这对 Jurkat 和人类原代 CD4 ⁺ T 细胞的体外 T 细胞活化反应没有重大影响。