Cloning and protein expression in heterologous systems are very useful tools for the study of viral proteins. In this work, an in vivo cloning strategy was applied using the yeast Saccharomyces cerevisiae, as an efficient and low-cost method to clone several cDNAs from the tilapia lake virus (TiLV). Samples of infected tilapia Oreochromis niloticus tissues were taken and used to isolate their RNA and to obtain and clone the ten viral cDNAs in a shuttle plasmid. The cloning efficiencies range from 5 to 100% but for seven of the cDNAs the values were above 40%, demonstrating the high efficiency of the method.

中文翻译：

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在面包酵母中使用体内策略有效克隆罗非鱼湖病毒互补 DNA

异源系统中的克隆和蛋白质表达是研究病毒蛋白质的非常有用的工具。在这项工作中，使用酵母酿酒酵母应用了体内克隆策略，作为从罗非鱼湖病毒 (TiLV) 克隆几种 cDNA 的有效且低成本的方法。采集受感染的罗非鱼Oreochromis niloticus组织样本，用于分离它们的 RNA，并在穿梭质粒中获得和克隆十个病毒 cDNA。克隆效率范围从 5% 到 100%，但对于 7 个 cDNA 的值高于 40%，证明了该方法的高效率。