ABSTRACT

The aim of this study was to adjust the basic culture medium for germination of pollen grains for the different species from the genus Hylocereus. Pollen grains from the species Hylocereus polyrhizus Weber and Hylocereus undatus (Haw.) Britton & Rose were withdrawn of anthers from flowers bagged the previous day, before its opening. Then, with a brush, the pollen grains were spread on the surface of Petri dishes containing 20 mL of culture medium. Four experiments were sequentially installed, considering and selecting the best results from the previous experiment to install the next one. Measurements were made on the length of pollen tube under different concentrations of boric acid. Moreover, germination rate readings were performed after 24 h. The pollen viability of species from the genus Hylocereus varies according to species and the pollen germination was influenced by composition and concentration of medium. The culture medium for the germination of pollen grains from H. polyrhizus species must be increased with 100 g L⁻¹ of sucrose, 518 mg L⁻¹ of calcium nitrate and 636 mg L⁻¹ of boric acid, the pH being measured for 5 and the medium solidified with 6 g L⁻¹ of agar. For H. undatus, the medium should be composed of 100 g L⁻¹ of sucrose, 616 mg L⁻¹ of calcium, 619 mg L⁻¹ of boric acid, 6 g L⁻¹ of agar and pH 6. Both species increased the length of pollen tube with the addition of boric acid.

摘要

这项研究的目的是调整基本的培养基，以适应Hylocereus属的不同种类的花粉颗粒的发芽。多花Hylocereus polyrhizus Weber和Hylocereus undatus种的花粉粒（山楂。）Britton＆Rose在开业前一天从袋装的花朵中取出了花药。然后，用刷子将花粉粒散布在含有20mL培养基的培养皿的表面上。依次安装了四个实验，考虑并从上一个实验中选择最佳结果以安装下一个。在不同浓度的硼酸下对花粉管的长度进行测量。此外，在24小时后进行发芽率读数。Hylocereus属物种的花粉生存力根据物种而异，并且花粉的萌发受培养基组成和浓度的影响。多毛H. polyrhizus花粉粒萌发的培养基必须用100 g L ^-1的蔗糖，518 mg L ^-1的硝酸钙和636 mg L ^-1的硼酸增加细菌的种类，测量pH值为5，并用6 g L ^-1的琼脂固化培养基。对于H. undatus，培养基应由100 g L ^-1的蔗糖，616 mg L ^-1的钙，619 mg L ^-1的硼酸，6 g L ^-1的琼脂和pH 6组成。两种物质均增加花粉管的长度加上硼酸。