The role of medial septum in the genesis of slow-wave sleep and the inhibition of rapid eye movement sleep has been established using neurotoxic lesion and chemical stimulation of the medial septum. Intracerebroventricular injection of endocannabinoids (anandamide) decreases wake and increases slow-wave and rapid eye movement sleep in rats. Central cannabinoid (CB1) receptors are localized in the rat medial septum; however, the role of cannabinoid receptors at the medial septum on the regulation of sleep-wakefulness in rats lacks evidence. In this study, we have examined the changes in sleep architecture of 21 male Wistar rats, divided into three groups. Initially, 6 rats were used for dose standardization. Subsequently, one group (n = 6) was microinjected with CB1 receptor agonist, R-(+)-WIN 55,212–2 mesylate salt, the second group (n = 6) received microinjection of CB1 receptor antagonist LY 320,135, and the third group (n = 5) was microinjected with the vehicle, DMSO at the medial septum using stereotaxy. The sleep-wake cycle was recorded using electroencephalogram, electro-oculogram, and electromyogram. Microinjection of CB1 receptor agonist at the medial septum decreased slow-wave sleep and increased total sleep time. The increase in total sleep time was due to an increased percentage of rapid eye movement sleep. After the third and fourth hour of CB1 receptor antagonist microinjection at the medial septum, slow-wave sleep decreased when compared to vehicle injection, while rapid eye movement sleep decreased compared to baseline. We conclude that the endocannabinoid system at the septal nucleus acts through CB1 receptors to increase rapid eye movement sleep in rats.

内侧隔膜在慢波睡眠发生中的作用以及快速眼动睡眠的抑制作用已经通过使用神经毒性病变和内侧隔膜的化学刺激得以确立。脑室内注射内源性大麻素（anandamide）可降低大鼠的觉醒，并增加大鼠的慢波和快速眼动睡眠。中枢大麻素（CB1）受体位于大鼠中隔中。然而，缺乏中隔膜上的大麻素受体在调节大鼠睡眠-觉醒方面的作用。在这项研究中，我们检查了分为三组的21只雄性Wistar大鼠的睡眠结构变化。最初，使用6只大鼠进行剂量标准化。随后，一组（Ñ = 6）显微注射CB1受体激动剂R-（+）-WIN 55,212–2甲磺酸盐，第二组（n  = 6）显微注射CB1受体拮抗剂LY 320,135，第三组（n ＝ 5）使用立体定位在内侧隔膜向媒介物DMSO显微注射。使用脑电图，眼电图和肌电图记录睡眠-觉醒周期。在中隔处显微注射CB1受体激动剂可减少慢波睡眠并增加总睡眠时间。总睡眠时间的增加是由于快速眼动睡眠的百分比增加。在中隔间隔第三和第四小时的CB1受体拮抗剂显微注射后，与媒介物注射相比，慢波睡眠减少，而与基线相比，快速眼动睡眠减少。我们得出的结论是，隔核中的内源性大麻素系统通过CB1受体起作用，从而增加了大鼠的快速眼动睡眠。