Abstract

In this work, we fabricated scaffolds for epithelial cell differentiation based on functional fibers. Chitosan, PCL, and PLLA composite scaffolds were electrospun under two controlled atmospheres. SEM results have shown yarns (<300 nm), strings (∼500 nm), and ribbons (∼2 µm). DSC and TGA indicate the main melting temperature ∼61 °C and a degradation temperature at 277 °C. Aqueous swell and UV sterilization (10 min) processes were tested before performing epithelial cell cultures over selected electrospun substrates. Propagation of the immortalized human keratinocyte cell line HaCaT (24 h) on scaffolds, its non-cytotoxic effect (95% relative to control), and cell differentiation characteristics were observed.

摘要

在这项工作中，我们制造了基于功能纤维的上皮细胞分化支架。壳聚糖、PCL 和 PLLA 复合支架在两种受控气氛下进行静电纺丝。SEM 结果显示纱线（<300 nm）、细绳（~500 nm）和丝带（~2 µm）。DSC 和 TGA 表明主要熔化温度为 ∼61 °C，降解温度为 277 °C。在对选定的电纺基质进行上皮细胞培养之前，测试了水溶胀和紫外线灭菌（10 分钟）过程。观察了永生化人角质形成细胞系 HaCaT（24 小时）在支架上的增殖、其非细胞毒性作用（相对于对照的 95%）和细胞分化特征。