simple, sensitive, free of organic solvents, kinetic spectrophotometric method has been developed for the determination of Clindamycin Hydrochloride, both in pure form and Capsules. Method is based on reaction of Clindamycin with potassium iodide and potassium iodate in an aqueous medium at (25 ± 2 °C) to produce yellow-coloured tri iodide ions (I3−). The reaction is followed spectrophotometrically by measuring the absorbance at wavelength 350 nm during 40 min. the effects of analytical parameters on reported kinetic methods were investigated. Under the optimized conditions, the initial rate and fixed time (at 10 min) methods were used for constructing the calibration graphs. The graphs were linear in concentration ranges 1–20 μg ml−1 with limit of detection of 0.12 and 0.22 μg ml−1for the initial rate and fixed time methods, respectively. The results were satisfactory and the analytical performance for both methods was validated. The proposed methods have been applied to determine the components in capsules with an average recovery of 98.25–102.00% and the results are in good agreement with those found by the reference method.

中文翻译：

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经验证的绿色分光光度动力学方法测定胶囊中盐酸克林霉素

开发了简单、灵敏、不含有机溶剂的动力学分光光度法测定盐酸克林霉素，包括纯品和胶囊剂。该方法基于克林霉素与碘化钾和碘酸钾在（25 ± 2 °C）水介质中反应生成黄色三碘离子（I3-）。通过在 40 分钟内测量波长 350 nm 处的吸光度，以分光光度法跟踪反应。研究了分析参数对报告的动力学方法的影响。在优化条件下，使用初始速率和固定时间（10 分钟）方法构建校准图。该图在浓度范围 1-20 μg ml-1 中呈线性，初始速率和固定时间方法的检测限分别为 0.12 和 0.22 μg ml-1。结果令人满意，两种方法的分析性能都得到了验证。所提出的方法已用于测定胶囊中的成分，平均回收率为 98.25-102.00%，结果与参考方法的结果非常吻合。