Cryobanking is a crucial part on species conservation. Nowadays, there is no suitable protocol for vitrification of feline oocytes. Self-pressurized rapid freezing of different cell types proved to mimic the advantages of high pressure freezing. As this method could also be applied for gamete rescue under field conditions, the aim here was to analyse the impact of self-pressurized vitrification on feline cumulus-oocyte-complexes (COCs) and to determine the appropriate material. Therefore, COCs of domestic cat were randomly vitrified (n = 189) in metal tubes of different materials: Aluminium, silver, and titanium. No significant differences were found on oocytes’ competence after thawing. On average, 44% of the COCs presented normal morphology and 48.2% of them showed a polar body after in vitro maturation (IVM) and were subsequently fertilised. Aluminium tubes were positive on toxicity tests, producing the lowest cleavage rates. Silver tubes showed no toxic effect, but the cleavage rate was lower than with titanium tubes, and a previous association with embryotoxicity and biological alterations makes us aware of its indiscriminate use. Titanium seems to be the only inert material of them, presenting a slightly higher maturation (55.6%) and cleavage (20%) rates. Nevertheless, more studies should follow to increase embryo competence after warming.

中文翻译：

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猫卵母细胞自加压玻璃化的不同材料的比较—初步结果

低温库是物种保护的关键部分。如今，没有合适的方案来对猫卵母细胞进行玻璃化。事实证明，不同细胞类型的自加压快速冷冻可以模仿高压冷冻的优势。由于该方法也可用于野外条件下的配子抢救，因此此处的目的是分析自加压玻璃化对猫卵丘-卵母细胞复合体（COC）的影响并确定合适的材料。因此，家猫的COC在不同材料的金属管（铝，银和钛）中随机玻璃化（n = 189）。解冻后卵母细胞的能力没有显着差异。平均而言，在体外成熟（IVM）后，有44％的COC表现出正常的形态，其中48.2％的表现为极体，随后受精。铝管在毒性测试中呈阳性，裂解率最低。银管没有显示毒性作用，但是其裂解率低于钛管，并且以前与胚胎毒性和生物学改变有关，这使我们意识到它的滥用。钛似乎是其中唯一的惰性材料，其成熟度（55.6％）和解理率（20％）略高。尽管如此，应进行更多的研究以增加变暖后的胚胎能力。呈现较高的成熟度（55.6％）和分裂（20％）比率。尽管如此，应进行更多的研究以增加变暖后的胚胎能力。呈现较高的成熟度（55.6％）和分裂（20％）率。尽管如此，应进行更多的研究以增加变暖后的胚胎能力。