ABSTRACT

The microbiota colonizing the rhizosphere contributes to plant growth, productivity, carbon sequestration and phytoremediation. Several studies address plant-associated bacteria; however, few studies analyze the effect of plant genotype on the eukaryotic community. Here, we analyzed the eukaryotic composition of maize rhizosphere from three different plant landraces and one inbred line grown in the same soil (common garden approach). This experimental design, coupled with 18S rDNA gene amplicon sequencing, allowed us to test the influence of maize and its genotype on the rhizosphere's eukaryotic community. We found that plant growth modified the eukaryotic community in soil, as diversity comparisons between maize rhizosphere and unplanted soil revealed significantly different eukaryotic composition. Various genera of nematodes and fungi, predominantly bacterial feeding nematodes and mycorrhizal fungi among other taxa, were increased in the rhizosphere samples. We also observed that maize genotype differentially shaped the relative abundance of the following fungal families in the rhizosphere: Acaulosporaceae, Aspergillaceae, Chaetomiaceae, Claroideoglomeraceae, Corticiaceae, Mortierellaceae, Trichocomaceae and Trichomeriaceae. Thus, plant genotype has a selective influence on establishing fungal communities in the rhizosphere. This study emphasizes the importance of an integrated consideration of plant genetics for future agricultural applications of microbes to crops.

中文翻译：

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玉米基因型对根际真核生物群落的影响

摘要

定植于根际的微生物群有助于植物生长、生产力、碳固存和植物修复。几项研究涉及植物相关细菌；然而，很少有研究分析植物基因型对真核生物群落的影响。在这里，我们分析了来自三种不同植物地方品种和同一土壤中生长的一种自交系（常见花园方法）的玉米根际的真核组成。这种实验设计与 18S rDNA 基因扩增子测序相结合，使我们能够测试玉米及其基因型对根际真核生物群落的影响。我们发现植物生长改变了土壤中的真核生物群落，因为玉米根际和未种植土壤之间的多样性比较揭示了显着不同的真核生物组成。各种线虫和真菌属，根际样品中主要以细菌为食的线虫和菌根真菌在其他分类群中增加。我们还观察到，玉米基因型对根际以下真菌家族的相对丰度有差异：Acaulosporaceae、Aspergillaceae、Chaetomiaceae、Claroideoglomeraceae、Corticiaceae、Mortierellaceae、Trichocomaceae 和 Trichomeriaceae。因此，植物基因型对在根际建立真菌群落具有选择性影响。这项研究强调了综合考虑植物遗传学对未来微生物在作物上的农业应用的重要性。我们还观察到，玉米基因型对根际以下真菌家族的相对丰度有差异：Acaulosporaceae、Aspergillaceae、Chaetomiaceae、Claroideoglomeraceae、Corticiaceae、Mortierellaceae、Trichocomaceae 和 Trichomeriaceae。因此，植物基因型对在根际建立真菌群落具有选择性影响。这项研究强调了综合考虑植物遗传学对未来微生物在作物上的农业应用的重要性。我们还观察到，玉米基因型对根际以下真菌家族的相对丰度有差异：Acaulosporaceae、Aspergillaceae、Chaetomiaceae、Claroideoglomeraceae、Corticiaceae、Mortierellaceae、Trichocomaceae 和 Trichomeriaceae。因此，植物基因型对在根际建立真菌群落具有选择性影响。这项研究强调了综合考虑植物遗传学对未来微生物在作物上的农业应用的重要性。