The intestinal symbiotic bacteria of Aedes albopictus play a potential role in host resistance to insecticides. In this study, we sequenced the full‐length of 16S rRNA and analyzed the differences in the intestinal microbiota between deltamethrin‐resistant and ‐sensitive Ae. albopictus. Symbiotic bacteria were cultured and analyzed using six types of culture media in aerobic and anaerobic environments. We found significant differences in the diversity and abundance of the intestinal microbiota of the two strains of Ae. albopictus. The symbiotic bacteria cultured in vitro were found to be mainly facultative anaerobes. The cultured bacteria such as Serratia oryzae and Acinetobacter junii may function to promote the development of insecticide resistance. This work indicates that intestinal bacteria may contribute to the enhancement of insecticide resistance of Ae. albopictus It also highlights the analytical advantage of full‐length 16S rRNA sequencing to study the intestinal microbiota of mosquitoes.

中文翻译：

---

基于全长16S rRNA测序的抗杀虫剂和敏感白纹伊蚊肠道微生物群的差异

白纹伊蚊的肠道共生菌在宿主对杀虫剂的抗性中发挥潜在作用。在这项研究中，我们对 16S rRNA 的全长进行了测序，并分析了溴氰菊酯抗性和敏感Ae之间肠道微生物群的差异。白线虫。在需氧和厌氧环境中使用六种培养基培养和分析共生细菌。我们发现两种Ae菌株的肠道微生物群的多样性和丰度存在显着差异。白线虫。发现体外培养的共生菌主要是兼性厌氧菌。米沙雷菌、六月不动杆菌等培养菌可能起到促进杀虫剂抗性发展的作用。这项工作表明肠道细菌可能有助于增强Ae的杀虫剂抗性。albopictus它还突出了全长 16S rRNA 测序在研究蚊子肠道微生物群方面的分析优势。