Dynamic changes occur in the DNA gut virome of female cynomolgus macaques during aging,MicrobiologyOpen

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Dynamic changes occur in the DNA gut virome of female cynomolgus macaques during aging
MicrobiologyOpen ( IF 3.9 ) Pub Date : 2021-05-01 , DOI: 10.1002/mbo3.1186
Xunmin Tan _{1,

2} , Tingjia Chai _{2,

3} , Jiajia Duan _{2,

4} , Jing Wu _{2,

4} , Hanping Zhang _{1,

2} , Yifan Li _{1,

2} , Yu Huang _{1,

2} , Xi Hu ₁ , Peng Zheng _{1,

2} , Jinlin Song _{5,

6,

7} , Ping Ji _{5,

6} , Xin Jin _{6,

8} , Hongmei Zhang _{5,

6} , Peng Xie _{1,

2}

Affiliation

Aging is a critical factor affecting physical health and disease in mammals. Emerging evidence indicates that aging may affect the gut bacteriome in cynomolgus macaques, but little is known about whether or how the gut virome changes with age. Here, we compared the DNA gut viral composition of 16 female cynomolgus monkeys (Macaca fascicularis) at three life stages (young, adult, and old) using the shotgun metagenome sequencing method. We found that the DNA gut virome from these monkeys differed substantially among the three groups. The gut viruses were dominated by bacteriophages, the most abundant of which was the Caudovirales order (i.e., Siphoviridae, Myoviridae, and Podoviridae families). Additionally, the co‐occurrence analysis revealed that the age‐related bacteriophages were correlated in an extensive and complex manner with the main intestinal bacteria (i.e., Firmicutes, Bacteroidetes, Proteobacteria, and Actinobacteria phyla). Furthermore, the age‐related DNA gut viral functions were enriched for genetic information processing, nucleotide, and folate metabolism. Our gut virome analysis provides new insight into how aging influences the gut virome of non‐human primates.

中文翻译：

雌性食蟹猴DNA肠道病毒组在衰老过程中发生的动态变化

衰老是影响哺乳动物身体健康和疾病的关键因素。新出现的证据表明，衰老可能会影响食蟹猴的肠道细菌群，但人们对肠道病毒群是否或如何随年龄变化知之甚少。在这里，我们使用鸟枪宏基因组测序方法比较了 16 只雌性食蟹猴 ( Macaca fascicularis ) 在三个生命阶段（年轻、成年和年老）的 DNA 肠道病毒组成。我们发现来自这些猴子的 DNA 肠道病毒组在三组中存在很大差异。肠道病毒以噬菌体为主，其中最丰富的是尾病毒目（即Siphoviridae、Myoviridae和Podoviridae家庭）。此外，共现分析表明，年龄相关的噬菌体与主要肠道细菌（即厚壁菌门、拟杆菌门、变形菌门和放线菌门）以广泛而复杂的方式相关。此外，与年龄相关的 DNA 肠道病毒功能被丰富用于遗传信息处理、核苷酸和叶酸代谢。我们的肠道病毒组分析为衰老如何影响非人类灵长类动物的肠道病毒组提供了新的见解。

更新日期：2021-05-02

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