Antibiotic resistance is a snowballing international threat. Some of the antibiotics have lost their effectiveness due to overuse and underuse. Thus, there is an urgent need to tackle this global challenge, either by inhibiting the resistance mechanisms or by the development of new chemical entities. Thus, in the current study, the antibacterial activity of selected phytomolecules was investigated against bacterial strains, alone and in combination, with standard drugs. The antibacterial potential of these phytomolecules was explored using in vitro assays (microtiter assay, bacterial growth kinetics, percentage retardation of growth, and antimicrobial synergy study) and in vivo studies (zebrafish infection model). In vitro and in vivo studies have shown promising antibacterial effects against, both, Gram-positive and Gram-negative bacteria. Moreover, a cell viability assay also indicated the cytoprotective effect of these phytomolecules in combination with standard antibiotics (SABX). Thus, these phytomolecules could be a promising broad-spectrum antibacterial agent in combination with standard antibiotics.

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选定植物分子的抗菌潜力：一项实验研究

抗生素耐药性是滚雪球般的国际威胁。一些抗生素由于过度使用和使用不足而失去了效力。因此，迫切需要通过抑制耐药机制或开发新的化学实体来应对这一全球挑战。因此，在目前的研究中，选定的植物分子对细菌菌株的抗菌活性进行了研究，单独或与标准药物联合使用。使用体外试验（微量滴定试验、细菌生长动力学、生长阻滞百分比和抗菌协同研究）和体内试验（斑马鱼感染模型）探索了这些植物分子的抗菌潜力。体外和体内研究已经显示出对革兰氏阳性菌和革兰氏阴性菌的有希望的抗菌作用。此外，细胞活力测定还表明这些植物分子与标准抗生素 (SABX) 组合的细胞保护作用。因此，这些植物分子与标准抗生素相结合可能是一种有前途的广谱抗菌剂。