Aedes vexans (Meigen) (Diptera: Culicidae) is a common mosquito species with worldwide distribution and it is a potential vector of various pathogens such as West Nile, Rift Valley, and Zika viruses, and Dirofilaria nematodes. Also due to its abundance, Ae. vexans could be a key species in determining new outbreaks of these important pathogens outside their present geographical range. Herein, we report on studies carried out in the laboratory to ascertain the suitability of the species for control strategies based on the exploitation of the endosymbiotic bacterium Wolbachia, known to induce reproductive incompatibility and/or virus resistance in the hosts. Aedes vexans showed low propensity to mate in narrow places, low male survival, and reduced hatchability of the eggs collected on artificial devices. Embryonic and adult transinfection with various Wolbachia strains were attempted. Whereas the first approach was barely tolerated by Ae. vexans eggs, transinfected progeny was obtained by adult microinjection using wAlbA and wAlbB Wolbachia but both strains were not efficiently transmitted vertically, leading to the loss of the infection by three generations. The present study highlights the low suitability of Ae. vexans to the infection by this bacterium and to the mass-rearing conditions generally required for Wolbachia-based control strategies.

中文翻译：

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关于伊蚊对基于沃尔巴克氏体的控制策略的适用性

Aedes vexans (Meigen) (Diptera: Culicidae) 是一种常见的蚊子物种，分布于世界各地，是西尼罗河病毒、裂谷病毒和寨卡病毒以及Dirofilaria线虫等各种病原体的潜在载体。也由于其丰富，Ae。vexans可能是确定这些重要病原体在其当前地理范围之外新爆发的关键物种。在此，我们报告了在实验室中进行的研究，以确定该物种是否适合基于内共生细菌Wolbachia 的控制策略，该细菌已知会导致宿主的生殖不相容性和/或病毒抗性。白纹伊蚊在狭窄的地方交配的倾向低，雄性存活率低，人工装置收集的卵孵化率降低。尝试用各种Wolbachia菌株进行胚胎和成体转染。而Ae几乎不能容忍第一种方法。在 vexans卵中，使用w AlbA 和w AlbB Wolbachia通过成人显微注射获得转染的后代，但这两种菌株都不能有效地垂直传播，导致感染消失了三代。本研究强调了Ae的低适用性。刺扰伊蚊的感染由该细菌，并通常需要用于大量饲养条件沃尔巴克氏体基于控制策略。