A 12-week feeding trial with Asian sea bass Lates calcarifer juveniles was conducted to evaluate the response of feeding defatted Superworm (Zophobas morio) larval meal on growth performance, feed utilization, apparent digestibility coefficient of nutrients and haemato-biochemical status for Asian sea bass. Five isonitrogenous (450 g/kg) and isolipidic (125 g/kg) experimental diets were formulated. A basal diet that contained no insect meal from defatted Superworm (DFSM) served as the control. Four diets were formulated where DFSM was included at 30 g/kg; 11.11% (DFSM-3), 60 g/kg; 22.22% (DFSM-6), 90 g/kg; 33.33% (DFSM-9) and 120 g/kg; 44.44% (DFSM-12), respectively, on a dry matter basis. All essential amino acids (EAA) except methionine, phenylalanine and threonine in DFSM compared favourably with fishmeal (FM) and arginine, histidine, isoleucine, leucine, tryptophan and valine were higher than that of FM. No significant (p > .05) differences in weight gain, specific growth rate and feed conversion ratio, hepato-somatic and viscera-somatic index were apparent among fish fed different levels of DFSM. Furthermore, no significant (p < .05) differences were found for apparent digestibility of dry matter (DM) and crude fat in response to dietary DFSM with different levels. A significant decreased linear relationship in the ADC of protein (p = .042) was noted as the level of DFSM incorporation increased in the experimental diet. No significant differences in haematocrit, MCHC and creatinine values were found among the experimental diets. However, a significant quadratic increase in haematocrit (p = .039) was detected as the level of DFSM increased in the diet. Serum cholesterol, alkaline phosphatase (ALP), glutamic pyruvic transaminase (GPT) and glutamic oxaloacetate transaminase (GOT) were linearly reduced with the increasing of dietary DFSM (linear, p = .043; p = .023; p = .018; p = .028). The findings of the present study indicated that growth, feed efficiency and haemato-biochemical indices were not adversely affected by partial substitution of FM up to 120 g/kg dietary inclusion of Superworm meal. We discuss this in the context of aquafeed applications for marine fish production in Asia for more sustainable production using novel protein ingredients and wider implications.

中文翻译：

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Superworm (Zophobas morio) 幼虫粉作为亚洲鲈鱼幼鱼实验日粮中部分蛋白质来源的初步评估

进行了为期 12 周的亚洲鲈鱼Lates calcarifer幼鱼饲养试验，以评估饲喂脱脂超级蠕虫（Zophobas morio) 幼鱼粉对亚洲鲈鱼生长性能、饲料利用率、营养物质表观消化率和血液生化状态的影响。配制了五种异氮 (450 g/kg) 和异脂 (125 g/kg) 实验饮食。不含来自脱脂超级蠕虫 (DFSM) 的昆虫粉的基础饮食用作对照。配制了四种日粮，其中 DFSM 的含量为 30 g/kg；11.11% (DFSM-3)，60 克/公斤；22.22% (DFSM-6)，90 克/公斤；33.33% (DFSM-9) 和 120 g/kg；分别为 44.44% (DFSM-12)，以干物质计。DFSM中除蛋氨酸、苯丙氨酸和苏氨酸外的所有必需氨基酸(EAA)均优于鱼粉(FM)，精氨酸、组氨酸、异亮氨酸、亮氨酸、色氨酸和缬氨酸均高于FM。无显着性 ( p > .05) 饲喂不同 DFSM 水平的鱼在增重、比生长率和饲料转化率、肝-体和内脏-体指数方面存在明显差异。此外， 对于不同水平的日粮 DFSM，干物质 (DM) 和粗脂肪的表观消化率没有发现显着差异 ( p < .05)。随着 实验饮食中 DFSM 掺入水平的增加，蛋白质 ADC 的线性关系显着降低 ( p = .042)。在实验饮食中没有发现血细胞比容、MCHC 和肌酐值的显着差异。然而，血细胞比容呈显着的二次增加 ( p = .039）随着饮食中 DFSM 水平的增加而检测到。血清胆固醇，碱性磷酸酶（ALP），谷丙转氨酶（GPT）和谷氨酸草酰乙酸转氨酶（GOT）与膳食DFSM的增大（线性，进行线性地减少p  = 0.043 p  = 0.023; p  = 0.018; p  = .028）。本研究的结果表明生长、饲料效率和血液生化指标不受部分替代 FM 的不利影响，最高 120 g/kg 日粮包含 Superworm 粉。我们在亚洲海洋鱼类生产的水产饲料应用的背景下讨论了这一点，以便使用新型蛋白质成分和更广泛的影响进行更可持续的生产。