BACKGROUND:Powdery mildew affects grapevine growth and development and reduces grapevine fruit yield and quality. Plant U-box (PUB) E3 ubiquitin ligases play important roles in ubiquitin/proteasome-mediated protein degradation during plant development and in the plant defense response. OBJECTIVE:Wecloned the VvPUB17 gene from Vitis vinifera and analyzed that VvPUB17 enhanced the resistance of grapevine to powdery mildew through the SA signal pathway. METHODS:Pathogen inoculation of Arabidopsis thaliana and grapevine plants was carried out by the tableting method. Gene expression was analyzed by quantitative real-time PCR. Sequence analysis and in vitro ubiquitination experiments show the structure and characteristics of VvPUB17. Exogenous salicylic acid, methyl jasmonate, ethylene and powdery mildew induced the expression of VvPUB17 in Arabidopsis leaves to verify the resistance of VvPUB17 to powdery mildew. RESULTS:Sequence analysis and in vitro ubiquitination experiments show that VvPUB17 contains U-box and Armadillo repeats (ARM repeat) and has E3 ubiquitin ligase activity dependent on the conserved U-box motif. Transgenic plants showed elevated levels of key genes related to the SA defense response pathway and high concentrations of salicylic acid. CONCLUSIONS:VvPUB17 functions as an E3 ubiquitin ligase that enhances the resistance of grapes to powdery mildew through the SA signal pathway.

中文翻译：

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葡萄VvPUB17充当E3泛素连接酶，并通过水杨酸信号通路增强白粉病抗性

背景：粉状霉菌影响葡萄的生长发育并降低葡萄果实的产量和品质。植物U-box（PUB）E3泛素连接酶在植物发育过程中的泛素/蛋白酶体介导的蛋白质降解以及植物防御反应中起重要作用。目的：从葡萄中分离出VvPUB17基因，并分析了VvPUB17通过SA信号途径增强了葡萄对白粉病的抗性。方法：采用压片法对拟南芥和葡萄植物进行病原菌接种。通过定量实时PCR分析基因表达。序列分析和体外泛素化实验显示了VvPUB17的结构和特征。外源水杨酸，茉莉酸甲酯，乙烯和白粉病诱导了拟南芥叶片中VvPUB17的表达，从而验证了VvPUB17对白粉病的抗性。结果：序列分析和体外泛素化实验表明，VvPUB17包含U-box和Armadillo重复序列（ARM重复序列），并具有E3泛素连接酶活性，这取决于保守的U-box基序。转基因植物显示出与SA防御反应途径和高浓度水杨酸相关的关键基因水平升高。结论：VvPUB17充当E3泛素连接酶，可通过SA信号途径增强葡萄对白粉病的抵抗力。序列分析和体外泛素化实验表明，VvPUB17包含U-box和犰狳重复序列（ARM重复序列），并且具有E3泛素连接酶活性，这取决于保守的U-box基序。转基因植物显示出与SA防御反应途径和高浓度水杨酸相关的关键基因水平升高。结论：VvPUB17充当E3泛素连接酶，可通过SA信号途径增强葡萄对白粉病的抵抗力。序列分析和体外泛素化实验表明，VvPUB17包含U-box和犰狳重复序列（ARM重复序列），并且具有E3泛素连接酶活性，这取决于保守的U-box基序。转基因植物显示出与SA防御反应途径和高浓度水杨酸相关的关键基因水平升高。结论：VvPUB17充当E3泛素连接酶，可通过SA信号途径增强葡萄对白粉病的抵抗力。