Botany, Ahead of Print.
Existing Cannabis sativa micropropagation protocols use a limited number of cultivars and the results are often not reproducible. Currently, Murashige and Skoog basal salt mixture (MS) + 0.5 μmol/L thidiazuron (TDZ) has been reported as the optimal medium for nodal micropropagation, yet our preliminary studies with this medium have resulted in abnormal morphology and high mortality rates in multiple cultivars. Following an initial screen of basal salt mixtures [MS, B5, BABI, and Driver–Kuniyaki–Walnut (DKW)], we determined that DKW produced the healthiest plants. In a second experiment, the multiplication rate and canopy area of explants grown on MS + 0.5 μmol/L TDZ and DKW + 0.5 μmol/L TDZ were compared using five drug-type cultivars. The combined multiplication average of explants grown on DKW + 0.5 μmol/L TDZ was 1.5× higher than explants grown on MS + 0.5 μmol/L TDZ. Similarly, the combined average of the canopy area was twice as large on DKW + 0.5 μmol/L TDZ. In the third experiment, callogenesis was compared using a concentration range of 2,4-dichlorophenoxyacetic acid (0–30 μmol/L) on both MS and DKW, and similarly, callus growth was superior on DKW. This study presents the largest comparison of basal salt compositions on the micropropagation of five commercially grown Cannabis cultivars to date.

中文翻译：

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与MS基础盐相比，DKW基础盐在多种商业化大麻品种中改善了微繁殖和call生

植物学，提前印刷。
现有的大麻苜蓿微繁殖方案使用的栽培品种数量有限，其结果通常不可重现。目前，已经报道了Murashige和Skoog基础盐混合物（MS）+ 0.5μmol/ L噻唑隆（TDZ）作为淋巴结微繁殖的最佳培养基，但是我们对这种培养基的初步研究导致多种形态的形态异常和高死亡率。在初步筛选基础盐混合物[MS，B5，BABI和Driver–Kuniyaki–Walnut（DKW）]之后，我们确定DKW生产出最健康的植物。在第二个实验中，使用五个药物类型的品种比较了在MS + 0.5μmol/ L TDZ和DKW + 0.5μmol/ L TDZ上生长的外植体的繁殖率和冠层面积。在DKW + 0.5μmol/ L TDZ上生长的外植体的组合平均乘积为1。比在MS + 0.5μmol/ L TDZ上生长的外植体高5倍。同样，在DKW + 0.5μmol/ L TDZ上，冠层面积的合并平均值是原来的两倍。在第三个实验中，使用2,4-二氯苯氧基乙酸（0–30μmol/ L）的浓度范围对MS和DKW上的愈伤组织进行了比较，同样，DKW上愈伤组织的生长也更好。这项研究提出了迄今为止，基础盐成分在五个商业化种植的大麻品种的微繁中的最大比较。