This study aimed at determining how the degradation of cereal non-starch polysaccharides (NSP) by dietary enzymes during feed digestion can influence nutrient digestibility and NSP fermentability in broilers. Ninety-six one-day-old male broilers were assigned to 4 different treatments: control and enzyme-supplemented wheat-based (WC, WE) or maize-based (MC, ME) treatments. Enzyme supplementation with endo-xylanase and endo-glucanase occurred from day 20 onwards. On day 28, digesta samples were collected. Nutrient digestibility, NSP recovery, oligosaccharide profile, and short-chain fatty acids (SCFA) content were determined. Enzyme supplementation in WE resulted in a higher starch (3%; p = 0.004) and protein (5%; p = 0.002) digestion in the ileum compared to WC. Xylanase activity in WE led to in situ formations of arabinoxylan-oligosaccharides consisting of 5 to 26 pentose units in the ileum. This coincided with decreased arabinose (p = 0.059) and xylose (p = 0.036) amounts in the ceca and higher acetate (p = 0.014) and butyrate (p = 0.044) formation in WE compared to WC. Conversely, complete total tract recovery of arabinoxylan in MC and ME suggested poor maize NSP fermentability. Overall, enzyme action improved nutrient digestibility and arabinoxylan fermentability in the wheat-based diet. The lower response of the maize-based diet to enzyme treatment may be related to the recalcitrance of maize arabinoxylan as well as to the high nutritive value of maize.

中文翻译：

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木聚糖酶和葡聚糖酶对肉鸡胃肠道低聚糖形成、碳水化合物发酵模式和养分利用的影响

本研究旨在确定饲料消化过程中日粮酶对谷物非淀粉多糖 (NSP) 的降解如何影响肉鸡的营养物质消化率和 NSP 发酵能力。96 只一日龄雄性肉鸡被分配到 4 个不同的处理组：对照和添加酶的基于小麦（WC、WE）或基于玉米（MC、ME）的处理组。从第20天开始补充内切木聚糖酶和内切葡聚糖酶。第28天，收集消化物样品。测定了养分消化率、NSP 回收率、寡糖谱和短链脂肪酸 (SCFA) 含量。与 WC 相比，WE 中补充酶导致回肠中淀粉（3%；p = 0.004）和蛋白质（5%；p = 0.002）的消化更高。WE 中的木聚糖酶活性导致回肠中原位形成由 5 至 26 个戊糖单元组成的阿拉伯木聚糖寡糖。与 WC 相比，这与盲肠中阿拉伯糖 ( p = 0.059) 和木糖 ( p = 0.036) 量的减少以及 WE 中乙酸盐 ( p = 0.014) 和丁酸盐 ( p = 0.044) 形成量的增加相一致。相反，MC 和 ME 中阿拉伯木聚糖的完全全道回收表明玉米 NSP 发酵能力较差。总体而言，酶作用提高了小麦饮食中的营养物质消化率和阿拉伯木聚糖发酵能力。以玉米为主的饮食对酶处理的较低反应可能与玉米阿拉伯木聚糖的顽抗性以及玉米的高营养价值有关。