Akkermansia muciniphila, as a mucin-degrading bacterium of the phylum Verrucomicrobia, associates with several disease states for its' concentration in the human intestinal tract. To accuracy and rapidly measure the concentration of A. muciniphila would be a promising candidate for rapid diagnosis of these diseases. Herein, we present a new method to label-free acquire the concentration of A. muciniphila by designing a graphene-based transistor. Relative permittivities of the transistor were varied by adding A. muciniphila with different concentrations. Various conductance changes, which were recorded by the graphene-based transistor, were generated, and, in turn, characterized the concentrations of A. muciniphila. The experimental results indicated that the approach can significantly distinguish different concentrations of A. muciniphila and has certain fault tolerance when the samples containing a small amount of other intestinal bacteria and impurities. The work offers a general measurement method of the concentration of A. muciniphila, and provide a novel and promising method for the diagnosis of several diseases associating with A. muciniphila.

中文翻译：

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用于疾病诊断的石墨烯晶体管测量粘蛋白阿克曼氏菌的方法


阿克曼氏菌（Akkermansia muciniphila）是疣微菌门的一种粘蛋白降解细菌，其在人类肠道中的浓度与多种疾病状态相关。准确且快速地测量 A. muciniphila 的浓度将是快速诊断这些疾病的有希望的候选者。在此，我们提出了一种通过设计基于石墨烯的晶体管来无标记获取 A. muciniphila 浓度的新方法。通过添加不同浓度的A. muciniphila来改变晶体管的相对介电常数。产生了由基于石墨烯的晶体管记录的各种电导变化，进而表征了 A. muciniphila 的浓度。实验结果表明，该方法能够显着区分不同浓度的A. muciniphila，并且在样品中含有少量其他肠道细菌和杂质时具有一定的容错能力。该工作提供了A. muciniphila浓度的通用测量方法，并为诊断与A. muciniphila相关的几种疾病提供了一种新颖且有前景的方法。