Improvement of dye removal efficiency is beneficial to development of bioremediation for textile effluent. In this study, the effect of lignin model compounds (LMCs) and enzymatic/mild acidolysis lignin (EMAL) from rice straw as co-substrate on dye decolorization by Ganoderma lucidum EN2 were evaluated. Two azo dyes, Remazol Brilliant Violet 5R (RBV5) and Sunset Yellow (SY) were tested. Eight of the thirty tested LMCs could significantly improve both RBV5 and SY decolorization, including 3,4,5-trimethoxycinnamic acid, 4-hydroxycinnamic acid, 3,5-dimethoxy-4 -hydroxybenzaldehyde, 3-hydroxybenzoic acid, 3‑hydroxy-4-methoxycinnamic acid, guaiacol, acetosyringone, and 2,6-dimethoxyphenol. The decolorization of RBV5 (from 87.04% to 97.90%) and SY (from 62.41% to 97.40%) by G. lucidum EN2 could be efficiently enhanced in the presence of EMAL. The addition of EMAL could increase the decolorization of higher concentration of azo dyes which is more difficult to be decolorized alone. Noteworthy, laccase activity increased with the increasing amount of EMAL. EMAL showed a positive effect on fungal growth but a negligible effect on pH. These results indicated that EMAL may act as inducer to stimulate the laccase production, and the increase of laccase activity accelerated dye decolorization.

染料去除效率的提高有利于纺织废水生物修复的发展。在这项研究中，评估了木质素模型化合物 (LMC) 和稻草中酶促/温和酸解木质素 (EMAL) 作为共底物对灵芝EN2染料脱色的影响。测试了两种偶氮染料 Remazol Brilliant Violet 5R (RBV5) 和日落黄 (SY)。30 种测试的 LMC 中有 8 种可以显着改善 RBV5 和 SY 脱色，包括 3,4,5-三甲氧基肉桂酸、4-羟基肉桂酸、3,5-二甲氧基-4-羟基苯甲醛、3-羟基苯甲酸、3-羟基-4 -甲氧基肉桂酸、愈创木酚、乙酰丁香酮和 2,6-二甲氧基苯酚。RBV5（从87.04％至97.90％）和SY（从62.41％至97.40％）的由脱色灵芝EN2 可以在 EMAL 的存在下有效地增强。EMAL的加入可以提高较高浓度的偶氮染料的脱色能力，而这种染料单独脱色比较困难。值得注意的是，漆酶活性随着 EMAL 量的增加而增加。EMAL 显示出对真菌生长的积极影响，但对 pH 的影响可以忽略不计。这些结果表明EMAL可能作为诱导剂刺激漆酶的产生，并且漆酶活性的增加加速了染料脱色。