Cultivation of Agaricus bisporus is a large horticultural industry for many countries worldwide, where a single variety is almost grown exclusively. Mushroom virus X (MVX), a complex of multiple positive-sense single stranded RNA (ss(+)RNA) viruses, is a major pathogen of typical A. bisporus crops. MVX can manifest a variety of symptoms in crops and is highly infective and difficult to eradicate once established in host mycelium. Currently our knowledge regarding the molecular response of A. bisporus fruit bodies to MVX infection is limited. In order to study the response of different A. bisporus strains with different susceptibilities to MVX, we designed a model system to evaluate the in-vitro transmission of viruses in A. bisporus hyphae over a time-course, at two crucial phases in the crop cycle. The symptom expression of MVX in these varieties and the transcriptomic and proteomic response of fruit bodies to MVX-infection were examined. Transmission studies revealed the high potential of MVX to spread to uninfected mycelium yet not into the fruit bodies of certain strains in a crop. MVX affected colour and quality of multiple fruit bodies. Gene expression is significantly altered in all strains and between times of inoculation in the crop. Genes related to stress responses displayed differential expression. Proteomic responses revealed restriction of cellular signalling and vesicle transport in infected fruit bodies. This in-depth analysis examining many factors relevant to MVX infection in different A. bisporus strains, will provide key insights into host responses for this commercially important food crop.

双孢蘑菇的栽培是全球许多国家的大型园艺产业，几乎只种植单一品种。蘑菇病毒 X (MVX) 是多种正链单链 RNA (ss(+)RNA) 病毒的复合物，是典型双孢蘑菇作物的主要病原体。MVX 可在作物中表现出多种症状，并且一旦在寄主菌丝体中建立，传染性强且难以根除。目前，我们对双孢蘑菇子实体对 MVX 感染的分子反应的了解是有限的。为了研究具有不同敏感性的不同双孢菌菌株对 MVX 的反应，我们设计了一个模型系统来评估体外病毒在双孢杆菌中的传播在作物周期的两个关键阶段，菌丝在一段时间内。检查了这些品种中 MVX 的症状表达以及子实体对 MVX 感染的转录组学和蛋白质组学反应。传播研究揭示了 MVX 传播到未感染菌丝体但不会传播到作物中某些菌株的子实体中的高潜力。MVX 影响多个子实体的颜色和质量。在所有菌株中以及在作物中接种的时间间隔内，基因表达显着改变。与应激反应相关的基因表现出差异表达。蛋白质组学反应揭示了受感染子实体中细胞信号传导和囊泡运输的限制。这项深入分析检查了与不同双孢蘑菇中MVX 感染相关的许多因素 菌株，将为这种具有重要商业价值的粮食作物的宿主反应提供关键见解。