Decachlorobiphenyl (DCB) is one of the 209 polychlorinated biphenyls congeners characterized by its high toxicity and chemical stability. It is produced by industrial activities. A possible strategy to eliminate DCB is by bacterial degradation. The main objective of this study was to define the optimal conditions for biodegradation and bioaccumulation of DCB by Pseudomonas extremaustralis ADA-5 isolated from a worm intestine. Bacterial growth kinetics were determined in minimal medium with added biphenyl and DCB. By GC coupled to mass spectrometry, we found that the strain had the ability to degrade 9.75% of available DCB, using it as a carbon source and was able to accumulate 19.98% of this pollutant in biomass. Membrane lipids may be altered by DCB. Phosphatidylethanolamine (PE), phosphatidylglycerol (PG), and cardiolipin (CL) were identified by thin-layer chromatography as the membrane lipids of the cell. At 250 mg L⁻¹ of DCB in the culture medium, membranes showed a 30% decrease in the PE concentration, an 18% increase in the PG, and a 12% increase in CL. ADA-5 was able to catabolize DCB and may be used for bioremediation of highly chlorinated toxic compounds in soil.

十氯联苯（DCB）是209种多氯联苯同源物之一，其高毒性和化学稳定性很好。它是通过工业活动生产的。消除DCB的可能策略是通过细菌降解。本研究的主要目的是确定极端假单胞菌对DCB进行生物降解和生物富集的最佳条件。从蠕虫肠道中分离出的ADA-5。在添加联苯和DCB的基本培养基中测定细菌的生长动力学。通过GC与质谱联用，我们发现该菌株能够降解9.75％的可用DCB，并将其用作碳源，并且能够在生物质中累积19.98％的这种污染物。膜脂质可能会被DCB改变。通过薄层色谱法鉴定了磷脂酰乙醇胺（PE），磷脂酰甘油（PG）和心磷脂（CL）作为细胞的膜脂质。在培养基中DCB为250 mg L ^-1时，膜的PE浓度降低30％，PG升高18％，CL升高12％。ADA-5能够分解DCB，可用于土壤中高氯毒性化合物的生物修复。