Anguillid herpesvirus 1 (AngHV) is one of the vital pathogenic agents found in the wild and cultured eel populations, which has brought significant losses to eel culture industry in China. In this study, AngHV ORF95 was characterized. Bioinformatics analysis showed that ORF95 putatively encodes a structural protein that is homologous to hemagglutinin-esterase (HE) protein of infectious salmon anemia virus (ISAV). Temporal transcription and expression analysis indicated that ORF95 is a viral late gene. Subcellular localization analysis revealed that ORF95 was predominantly localized in the cytoplasm. Further, western blot analysis indicated that ORF95 is a structural protein of virion envelope. These results provide a novel basis to make further efforts to clarify the function of ORF95 in the process of AngHV infection and the possibility to use ORF95 as antigen to develop AngHV subunit vaccine.

鳗鱼疱疹病毒1（AngHV）是在野生和养殖鳗鱼种群中发现的重要病原体之一，给我国鳗鱼养殖业带来了重大损失。在这项研究中，AngHV ORF 95 被表征。生物信息学分析表明，ORF 95 可能编码一种结构蛋白，该蛋白与传染性鲑鱼贫血病毒 (ISAV) 的血凝素酯酶 (HE) 蛋白同源。时间转录和表达分析表明ORF95是病毒晚期基因。亚细胞定位分析表明，ORF95 主要定位于细胞质中。此外，蛋白质印迹分析表明ORF95是病毒体包膜的结构蛋白。这些结果为进一步阐明ORF95在AngHV感染过程中的作用以及利用ORF95作为抗原开发AngHV亚单位疫苗的可能性提供了新的基础。