Less attention had been paid to cell toxicity of the various synthesis methods of nanoparticles, this study investigated the effect of the calcination temperature(CT) on the crystallization of SiO₂ nanoparticles(NPs), cell proliferation(CP), and cellular uptake(CU) in MRC-5. In this study, parameters were adjusted as CT(70–1000 °C), calcination time(2, 12, and 24 h), and catalyst feed rate(0.01, 0.05, and 0.1 mL.min¹). CP was determined by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide(MTT) test after a 24-h exposure. The CU was achieved using ICP-MS. Results were analyzed using MATLAB2018. Results revealed that the size of synthesized particles was lower than 50 nm and, the XRD peak varied from 21 to 30° during the increase in CT. FTIR spectra confirmed the existence of Si-O and Si-Cl bonds. The maximum level of crystallization was at 1000 °C. CP decreased with the rise in the concentration of NPs(p < 0.05), as well as an increase in feed rate. A positive relationship between increased crystallization and decreased CP(R = 0.78) was seen, while such a trend was not observed in calcination time. The suggested structure in this study was 4:10:1 with R_all = 0.97, R_test = 0.97, RMSE = 0.25, and MSE = 0.003. Furthermore, the CU rate increased with the rise in CT and calcination time. The maximum and minimum CU levels were related to NPs calcinated in 1000 °C-24 h and 350 °C-2 h, respectively. As a consequence, the most toxicity of SiO₂ NPs was related to the crystalline NP. Therefore, the increase in CT and the calcination time were significant factors affecting on crystallization of SiO₂ NPs, CP of lung cell, as well as CU of SiO₂.

纳米粒子的各种合成方法对细胞毒性的关注较少，本研究考察了煅烧温度(CT)对SiO ₂纳米粒子(NPs)结晶、细胞增殖(CP)和细胞摄取(CU)的影响。 ) 在 MRC-5 中。在本研究中，参数调整为 CT（70-1000 ℃）、煅烧时间（2、12 和 24 h）和催化剂进料速率（0.01、0.05 和 0.1 mL.min ¹）。CP 是通过 3-(4,5-二甲基噻唑-2-基)-2,5-二苯基溴化四唑 (MTT) 测试在 24 小时暴露后测定的。CU 是使用 ICP-MS 实现的。使用 MATLAB2018 分析结果。结果表明，合成颗粒的尺寸小于50 nm，并且随着CT的增加，XRD峰在21°到30°之间变化。FTIR 光谱证实了 Si-O 和 Si-Cl 键的存在。结晶的最高水平是在 1000 °C。CP随着NPs浓度的增加( p  < 0.05)以及进料量的增加而降低。观察到结晶增加和 CP 减少之间存在正相关关系（R = 0.78），而在煅烧时间中没有观察到这种趋势。本研究中建议的结构为 4:10:1，R _all  = 0.97，R_检验 = 0.97，RMSE = 0.25，MSE = 0.003。此外，CU 率随着 CT 和煅烧时间的增加而增加。最大和最小 CU 水平分别与 1000 °C-24 h 和 350 °C-2 h 煅烧的 NPs 有关。因此，SiO ₂ NPs 的最大毒性与结晶 NP 有关。因此，CT的增加和煅烧时间的增加是影响SiO ₂ NPs结晶、肺细胞CP以及SiO ₂ CU结晶的重要因素。