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Validation and Application of Ultra-Performance Liquid Chromatography–Tandem Mass Spectrometry Method for Aflatoxins and Ochratoxin A Evaluation in Argan Kernels and Edible Argan Oil from Morocco
ACS Food Science & Technology ( IF 2.6 ) Pub Date : 2021-04-29 , DOI: 10.1021/acsfoodscitech.1c00031 Abdallah Ouakhssase 1 , Noureddine Fatini 2 , Elhabib Ait Addi 1
ACS Food Science & Technology ( IF 2.6 ) Pub Date : 2021-04-29 , DOI: 10.1021/acsfoodscitech.1c00031 Abdallah Ouakhssase 1 , Noureddine Fatini 2 , Elhabib Ait Addi 1
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Argan fruit and argan oil are endemic products from Morocco and are like other oleaginous fruits with edible oils. However, there are no published data to indicate whether mycotoxin could contaminate argan kernels and edible argan oil. The study of the contamination of this product by mycotoxins is essential. In our study, all collected samples (argan fruits and argan oil) were stored for a long period (approximately 20 months) in conditions that were probably conducive to the development of molds. For quantitative analysis of mycotoxins, a sensitive ultraperformance liquid chromatography–tandem mass spectrometry method is proposed. Aflatoxin B1 (AFB1), aflatoxin B2 (AFB2), aflatoxin G1 (AFG1), aflatoxin G2 (AFG2), and ochratoxin A (OTA) were extracted using a modified QuEChERS method. The extracts were frozen overnight (−18 °C) to separate the majority of lipids and subjected to a cleanup step with C18 as dispersing material. The method was successfully validated in kernel powder and argan oil. Good linearity (0.125–20 μg/kg, R2 > 0.99), limits of quantification (0.45–0.8 μg/kg), recoveries (78–112%), and precision (<11%) were achieved for the five mycotoxins. Matrix-matched calibration curves were employed for accurate quantification. Using this method, no mycotoxins were found above the limit of quantification in any of the analyzed samples.
中文翻译：
摩洛哥坚果仁和食用摩洛哥坚果油中黄曲霉毒素和赭曲霉毒素 A 评估的超高效液相色谱-串联质谱法的验证和应用
摩洛哥坚果和摩洛哥坚果油是摩洛哥的特有产品，与其他含油水果一样含有食用油。然而，并没有公布的数据表明霉菌毒素是否会污染摩洛哥坚果仁和食用摩洛哥坚果油。研究霉菌毒素对本产品的污染是必不可少的。在我们的研究中，所有收集的样品（摩洛哥坚果油和摩洛哥坚果油）都在可能有利于霉菌生长的条件下储存了很长时间（大约 20 个月）。对于真菌毒素的定量分析，提出了一种灵敏的超高效液相色谱-串联质谱法。使用改进的 QuEChERS 方法提取黄曲霉毒素 B1 (AFB1)、黄曲霉毒素 B2 (AFB2)、黄曲霉毒素 G1 (AFG1)、黄曲霉毒素 G2 (AFG2) 和赭曲霉毒素 A (OTA)。18作为分散材料。该方法在仁粉和摩洛哥坚果油中得到了成功验证。五种真菌毒素均实现了良好的线性（0.125–20 µg/kg，R 2 > 0.99）、定量限（0.45–0.8 µg/kg）、回收率（78–112%）和精密度（<11%）。采用基质匹配校准曲线进行准确定量。使用这种方法，在任何分析的样品中都没有发现超过定量限的霉菌毒素。
更新日期：2021-06-18
中文翻译：
摩洛哥坚果仁和食用摩洛哥坚果油中黄曲霉毒素和赭曲霉毒素 A 评估的超高效液相色谱-串联质谱法的验证和应用
摩洛哥坚果和摩洛哥坚果油是摩洛哥的特有产品，与其他含油水果一样含有食用油。然而，并没有公布的数据表明霉菌毒素是否会污染摩洛哥坚果仁和食用摩洛哥坚果油。研究霉菌毒素对本产品的污染是必不可少的。在我们的研究中，所有收集的样品（摩洛哥坚果油和摩洛哥坚果油）都在可能有利于霉菌生长的条件下储存了很长时间（大约 20 个月）。对于真菌毒素的定量分析，提出了一种灵敏的超高效液相色谱-串联质谱法。使用改进的 QuEChERS 方法提取黄曲霉毒素 B1 (AFB1)、黄曲霉毒素 B2 (AFB2)、黄曲霉毒素 G1 (AFG1)、黄曲霉毒素 G2 (AFG2) 和赭曲霉毒素 A (OTA)。18作为分散材料。该方法在仁粉和摩洛哥坚果油中得到了成功验证。五种真菌毒素均实现了良好的线性（0.125–20 µg/kg，R 2 > 0.99）、定量限（0.45–0.8 µg/kg）、回收率（78–112%）和精密度（<11%）。采用基质匹配校准曲线进行准确定量。使用这种方法，在任何分析的样品中都没有发现超过定量限的霉菌毒素。
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