Heterocyclic compound quinoline and its derivatives exist in natural compounds and have a broad spectrum of biological activity. They play an important role in the design of new structural entities for medical applications. Similarly, indoles and their derivatives are found widely in nature. Amino acids, alkaloids and auxin are all derivatives of indoles, as are dyes, and their condensation with aldehydes makes it easy to construct reaction sites for nucleophilic addition agents. In this work, we combine these two groups organically to construct a rapid response site (within 30 s) for H₂S, and at the same time, a ratiometric fluorescence response is presented throughout the process of H₂S detection. As such, the lower detection limit can reach 55.7 nmol/L for H₂S. In addition, cell imaging shows that this probe can be used for the mitochondrial targeted detection of endogenous and exogenous H₂S. Finally, this probe application was verified by imaging H₂S in nude mice.

杂环化合物喹啉及其衍生物存在于天然化合物中，并具有广泛的生物活性。它们在设计用于医疗应用的新结构实体中起着重要作用。类似地，吲哚及其衍生物在自然界中被广泛发现。氨基酸，生物碱和植物生长素与染料一样，都是吲哚的衍生物，它们与醛的缩合使得易于构建亲核加成剂的反应位点。在这项工作中，我们将这两个基团有机地结合起来，构建了一个对H ₂ S的快速响应位点（在30 s之内），同时，在整个H ₂ S检测过程中都呈现了比例荧光响应。因此，H _2的检测下限可以达到55.7 nmol / LS.另外，细胞成像显示，该探针可以被用于内源性和外源性H的线粒体靶向检测₂ S.最后，该探针应用程序是由成像ħ验证₂裸鼠S上。