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An improved high-throughput method for assessing the impact of bioformulation on plant parameters: Case study with pigeonpea seeds
Physiological and Molecular Plant Pathology ( IF 2.8 ) Pub Date : 2021-04-30 , DOI: 10.1016/j.pmpp.2021.101657
Sakshi Tewari , Shilpi Sharma

Pigeonpea is an important legume cultivated in India, owing to its high nutritive and protein value. Various eco-friendly and green strategies have been proposed by researchers for enhancing the productivity of this pulse crop. In the present study, different bioformulations were prepared from plant growth promoting (PGP) bacterial strains and coated on pigeonpea seeds for monitoring growth enhancement of plantlets under in vitro conditions. For performing initial in vitro experiments with efficient bioinoculants, the conventional methods employ either tubes or Petri dishes. Despite delivering satisfactory results under laboratory conditions these methods suffer from inherent limitations of being cumbersome, difficult, and error-prone when multiple samples are handled simultaneously. The present study aimed to develop a high-throughput method for recording plant parameters of more than ninety seeds simultaneously, thus facilitating the process. The study opens up avenues of faster assessment to answer a range of scientific questions related to plant-microbe interactions and beyond.



中文翻译:

一种改进的高通量方法,用于评估生物制剂对植物参数的影响:以木豆种子为例

木豆具有很高的营养价值和蛋白质价值,是印度种植的一种重要豆类。研究人员提出了各种生态友好和绿色战略,以提高这种豆类作物的生产力。在本研究中,从植物生长促进(PGP)细菌菌株制备了不同的生物制剂,并涂在木豆种子上,以监测体外条件下小植物的生长。用于进行初始体外为了进行有效的生物灭活剂实验,常规方法采用试管或培养皿。尽管在实验室条件下能获得令人满意的结果,但是当同时处理多个样品时,这些方法固有的局限性是麻烦,困难和容易出错。本研究旨在开发一种高通量方法,用于同时记录90多个种子的植物参数,从而简化了这一过程。该研究开辟了更快的评估途径,以回答与植物-微生物相互作用及其他相关的一系列科学问题。

更新日期:2021-05-08
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