Penicillium expansum is a pathogenic fungus that causes blue mold decay in various fruits and produces toxic substances. This study investigated the effect of alginate oligosaccharide (AOS) combined with Meyerozyma guilliermondii, a biocontrol yeast, to control blue mold decay in pears and the possible physiological biocontrol mechanisms. The results showed that AOS (5 g/L) combined with M. guilliermondii could significantly reduce the blue mold decay incidence and lesion diameter of pears. Moreover, AOS did not affect the growth and reproduction of M. guilliermondii in vitro or in vivo of pears. And could inhibit mycelium development, reduce spore germination rate and inhibit the germ tube elongation of P. expansum. AOS combined with M. guilliermondii increased the activities of superoxide dismutase (SOD), catalase (CAT), polyphenol oxidase (PPO), peroxidase (POD), phenylalanine ammonia-lyase (PAL), chitinase (CHI), total phenol content, and flavonoid content in pears. Overall, our study evidenced that AOS improves the efficacy of M. guilliermondii and could be used to control the blue mold decay in pears.

扩展青霉是一种致病性真菌，会导致各种水果的蓝霉变质并产生有毒物质。这项研究调查了藻酸盐低聚糖（AOS）与生物防治酵母菌Meyerozyma guilliermondii的结合对梨中蓝霉菌腐烂的控制作用以及可能的生理生物防治机制。结果表明，AOS（5 g / L）结合古拉蒙德莫氏菌可以显着降低梨的蓝霉变质发生率和病斑直径。而且，AOS在梨的体外或体内均不影响古拉氏莫尔氏菌的生长和繁殖。并能抑制菌丝体的发育，降低孢子萌发率，抑制胚芽的伸长。扩展体育。AOS与guilliermondii菌的结合增加了超氧化物歧化酶（SOD），过氧化氢酶（CAT），多酚氧化酶（PPO），过氧化物酶（POD），苯丙氨酸氨裂解酶（PAL），几丁质酶（CHI），总酚含量和梨中的类黄酮含量。总体而言，我们的研究表明AOS可以提高瓜地拉尔莫尔氏菌的功效，可用于控制梨的蓝霉菌腐烂。