A human hepatocarcinoma cell line, PLC/PRF/5, is susceptible to hepatitis E virus (HEV) infection and is used for isolating this virus. It is difficult to use this cell line for the isolation of HEV directly from fecal specimens of swine or wild boar contaminated with porcine sapelovirus (PSV), because PSV infection results in rapid and extensive cytopathic effects in PLC/PRF/5 cells, interrupting the growth of HEV. Herein, we used a PSV infection-resistant cell line, N1380 derived from PLC/PRF/5 cells, and we successfully isolated an HEV-4b strain from a PSV-positive swine fecal specimen. Our results indicate that N1380 cells are a useful tool for the isolation of HEV from swine or wild boar fecal specimens, even when they are co-infected with PSV.

人肝癌细胞系 PLC/PRF/5 易受戊型肝炎病毒 (HEV) 感染，并用于分离这种病毒。很难使用这种细胞系直接从被猪 sapelovirus (PSV) 污染的猪或野猪的粪便标本中分离 HEV，因为 PSV 感染会导致 PLC/PRF/5 细胞中的快速和广泛的细胞病变效应，中断HEV的增长。在此，我们使用源自 PLC/PRF/5 细胞的 PSV 抗感染细胞系 N1380，并成功从 PSV 阳性猪粪便标本中分离出 HEV-4b 菌株。我们的结果表明 N1380 细胞是从猪或野猪粪便标本中分离 HEV 的有用工具，即使它们与 PSV 共感染。