Synchronized research on endothelial dysfunction and microcirculation structure in dorsal skin of rats with type 2 diabetes mellitus,Medical & Biological Engineering & Computing

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Synchronized research on endothelial dysfunction and microcirculation structure in dorsal skin of rats with type 2 diabetes mellitus
Medical & Biological Engineering & Computing ( IF 2.6 ) Pub Date : 2021-04-29 , DOI: 10.1007/s11517-021-02363-5
Yajie Wei ₁ , Huimin Chen ₁ , Qingzhuo Chi ₁ , Ying He ₁ , Lizhong Mu ₁ , Chao Liu ₂ , Yunxia Lu ₂

Affiliation

Abstract

The aim of this study was to explore changes in the microvascular tone as measured by laser Doppler flowmetry (LDF) and the microcirculation structure of the dorsal skin of rats with type 2 diabetes mellitus. The diabetic rat model was induced by a diet of high-sugar and high-lipid fodder combined with the injection of streptozotocin into the abdominal cavity. Depending on the interval between the development of diabetes and the experiments, the diabetic rats were subdivided into three groups. The evaluation of microvascular tone was based on the amplitude responses of the LDF signal fluctuations in the appropriate frequency range in the dorsal skin of the rats during a thermal test (at 42 °C). The nitric oxide (NO) level in plasma was also used as a marker of endothelial dysfunction. Changes in the microcirculation structure in the diabetic rats were estimated by measuring the microvascular density in the choke vessels of the dorsal skin of the rats. The experimental results with respect to red blood cell (RBC)–related parameters showed decreased hematocrit and hemoglobin levels and increased standard deviation of the width of the RBC distribution in three diabetic rats. The increasing fluctuation amplitudes diminished in the endothelial frequency range in response to the thermal test and this was accompanied by abnormal NO levels in plasma of the diabetic groups as compared with healthy rats. A significant reduction in the microvascular density of the choke vessels of the dorsal skin was found only in the diabetic group at the most advanced stage of diabetes in this experiment. Thus, we suggest that endothelial dysfunction occurs in diabetic rats and changes in the microcirculation structure of the dorsal skin occur in a later stage of diabetes development.

Graphical abstract

A. Photograph of measurement method by using a LDF probe and heating device in the dorsal skin of the rat.

B. Dorsal skin LDF signals of a healthy rat during the thermal stimuli test. (a) Blood flow signal record for the test. Wavelet filtration of blood flow signal in (b) myogenic range, (c) neurogenic range, and (d) endothelial range

中文翻译：

2型糖尿病大鼠背部皮肤内皮功能障碍与微循环结构的同步研究

摘要

本研究的目的是探索通过激光多普勒血流仪 (LDF) 测量的微血管张力的变化和 2 型糖尿病大鼠背部皮肤的微循环结构。采用高糖高脂饲料联合链脲佐菌素腹腔注射诱导糖尿病大鼠模型。根据糖尿病发展与实验之间的时间间隔，将糖尿病大鼠细分为三组。微血管张力的评估基于热测试期间（42°C）大鼠背部皮肤适当频率范围内 LDF 信号波动的幅度响应。血浆中的一氧化氮 (NO) 水平也被用作内皮功能障碍的标志。通过测量大鼠背部皮肤阻塞血管中的微血管密度来估计糖尿病大鼠微循环结构的变化。关于红细胞 (RBC) 相关参数的实验结果显示，三只糖尿病大鼠的血细胞比容和血红蛋白水平降低，红细胞分布宽度的标准偏差增加。响应热测试，在内皮频率范围内增加的波动幅度减小，并且与健康大鼠相比，这伴随着糖尿病组血浆中的异常NO水平。在本实验中，仅在糖尿病最晚期阶段的糖尿病组中发现背部皮肤阻塞血管的微血管密度显着降低。因此，