Suppression of established populations of invasive alien species can be a complex and expensive process, which is frequently unsuccessful. The Asian clam, Corbicula fluminea (Müller, 1774), is considered a high impact invader that can adversely alter freshwater ecosystems and decrease their socioeconomic value. To date, C. fluminea continues to spread and persist within freshwater environments worldwide, despite repeated management attempts to prevent dispersal and suppress established populations. As extensive C. fluminea beds can often become exposed during low-water conditions, the direct application of hot or cold thermal shock treatments has been proposed as suitable mechanism for their control. Further, mechanical substrate disturbance may enhance the efficacy of thermal shock treatments by facilitating exposures to multiple layers of buried clams. In the present study, we advanced these methods by assessing combined applications of both hot and cold thermal shock treatments for control of C. fluminea, using steam spray (≥100 °C; 350 kPa), low- or high-intensity open-flame burns (~1000 °C) and dry ice (−78 °C). In a direct comparison of raking combined with hot thermal shock applications, both steam and high-intensity open-flame treatments tended to be most effective, especially following multiple applications. In addition, when hot thermal treatments are followed by a final cold shock (i.e. dry ice), steam treatments tended to be most effective. Further, when dry ice was applied either alone or prior to an application of a hot shock treatment, substantial if not complete C. fluminea mortality was observed. Overall, this study demonstrated that combined applications of hot and cold thermal shock treatments, applied following the disruption of the substrate, can substantially increase C. fluminea mortality compared to separate hot or cold treatments.

抑制外来入侵物种的既定种群可能是一个复杂而昂贵的过程，通常是不成功的。亚洲蛤Cor（Corbicula fluminea，Müller，1774年）被认为是一种高影响力的入侵者，可以不利地改变淡水生态系统并降低其社会经济价值。迄今为止，尽管反复进行管理以防止扩散和抑制已建立种群，但C. fluminea仍继续在全球淡水环境中传播并持续存在。作为广泛的C. fluminea床在低水条件下经常会暴露在外，因此建议直接应用热或冷热冲击处理作为控制它们的合适机制。此外，通过促进暴露于多层掩埋蛤layers，机械基底扰动可以增强热冲击处理的功效。在本研究中，我们通过评估热和冷热休克疗法在控制C. fluminea中的联合应用来改进这些方法。，使用蒸汽喷雾（≥100°C； 350 kPa），低强度或高强度明火燃烧（〜1000°C）和干冰（-78°C）。在将耙齿与热冲击相结合的直接比较中，蒸汽处理和高强度明火处理都趋于最有效，尤其是在多次应用之后。另外，当热热处理之后是最终的冷冲击（即干冰）时，蒸汽处理往往是最有效的。此外，当干冰单独使用或在热休克处理之前使用时，观察到相当大的C. fluminea死亡率。总体而言，这项研究表明，在基材破裂后进行的热和冷热冲击处理的组合应用可以大大增加与单独的热疗或冷疗相比，C。fluminea死亡率高。