The purpose of this article was to probe the mechanism underlying long noncoding RNA (lncRNA)-LINC00184 in cholangiocarcinoma development and to investigate the effects of LINC00184 on cholangiocarcinoma. We used bioinformatics to analyze the expression of LINC00184, microRNA (miR)-23b-3p and ANXA2 in cholangiocarcinoma tissues. The levels of LINC00184, miR-23b-3p, and ANXA2 were detected by qRT-PCR. Cell proliferation was tested by CCK8. Transwell assay was used to detect cell invasion and migration. The target connection between LINC00184, miR-23b-3p, or ANXA2 was probed by luciferase reporter assay. RNA pull-down method was employed to test the relationship among LINC00184/miR-23b-3p/ANXA2 in cholangiocarcinoma cells. The Pearson correlation coefficient analyzed was applied to analyze the correlation among LINC00184, miR-23b-3p, and ANXA2. LC–MS/M analysis was used to explore whether the changes of adenine metabolism was affected by LINC00184 in cholangiocarcinoma cells. We discovered that LINC00184 expression was heightened in cholangiocarcinoma patients and cells. Knockdown of LINC00184 repressed cell proliferation, invasion, migration and adenine metabolism in cholangiocarcinoma cells. miR-23b-3p was regarded as a target of LINC00184 and its depletion perversed the inhibitive influence of LINC00184 silencing on cholangiocarcinoma cells. ANXA2 was a target of miR-23b-3p and was negatively modulated by miR-23b-3p. Moreover, ANXA2 was positively modulated by LINC00184 via sponging miR-23b-3p. In short, silencing of LINC00184 suppressed cell proliferation, invasion and migration through over-expression of miR-23b-3p and reducing of ANXA2 in cholangiocarcinoma cells. These findings contribute to understanding the influences of LINC00184, miR-23b-3p, and ANXA2 on cholangiocarcinoma and provide basis for cholangiocarcinoma treatment.

中文翻译：

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长链非编码 RNA LINC00184 通过调节 hsa-miR-23b-3p/ANXA2 轴促进胆管癌的增殖、转移和腺嘌呤代谢

本文旨在探讨长链非编码RNA（lncRNA）-LINC00184在胆管癌发展中的作用机制，并探讨LINC00184对胆管癌的影响。我们使用生物信息学分析了胆管癌组织中 LINC00184、microRNA (miR)-23b-3p 和 ANXA2 的表达。通过qRT-PCR检测LINC00184、miR-23b-3p和ANXA2的水平。通过CCK8测试细胞增殖。Transwell测定用于检测细胞侵袭和迁移。LINC00184、miR-23b-3p 或 ANXA2 之间的靶标连接通过荧光素酶报告基因测定进行探测。采用RNA pull-down法检测胆管癌细胞中LINC00184/miR-23b-3p/ANXA2之间的关系。应用Pearson相关系数分析LINC00184、miR-23b-3p和ANXA2之间的相关性。LC-MS/M分析用于探索胆管癌细胞中腺嘌呤代谢的变化是否受到LINC00184的影响。我们发现胆管癌患者和细胞中 LINC00184 的表达升高。敲除 LINC00184 可抑制胆管癌细胞中的细胞增殖、侵袭、迁移和腺嘌呤代谢。miR-23b-3p 被认为是 LINC00184 的靶标，其消耗破坏了 LINC00184 沉默对胆管癌细胞的抑制作用。ANXA2 是 miR-23b-3p 的靶标，并受到 miR-23b-3p 的负调控。此外，LINC00184 通过海绵化 miR-23b-3p 正向调节 ANXA2。简而言之，LINC00184 的沉默通过 miR-23b-3p 的过表达和胆管癌细胞中 ANXA2 的减少来抑制细胞增殖、侵袭和迁移。