The antagonistic effect of Pseudomonas fluorescens (Pf) against soil borne fungal pathogen and reproductive toxicity of Pf to silkworm, Bombyx mori L. was studied. The antagonistic property of Pseudomonas fluorescens isolates viz., Pseudomonas fluorescens 5 (Pf 5), Pseudomonas fluorescens 18 (Pf 18) and Pseudomonas fluorescens 25 (Pf 25) was evaluated by in vitro dual culture method against soil-borne fungal pulse pathogens viz., Fusarium spp., Alternaria alternata, Macrophomina phaseolina and Rhizoctonia solani. Carbendazim 50% W.P. served as positive standard whereas fungal pathogen without antagonist (Pf) was maintained as control group. Mycelia growth was recorded in all the treatment groups and control. The results of different isolates were statistically compared by one-way ANOVA. The percentage mycelia growth inhibition over control was recorded in the order of Carbendazim > Pf 5 > Pf 25 > Pf 18 against the fungal pathogens tested. Toxicity to silkworm was evaluated by Leaf Dip Method. The treatments tested were Pf 5, Pf 18, Pf 25 @ 10⁸ cfu/mL and Dimethoate 30% EC was used as positive standard. Leaves dipped in deionized water served as a control. There is no significant difference recorded in treatments on mortality, larval duration and cocoon weight data compared to control. Feed consumption was also on par with the control. Therefore, the selected isolates were found to inhibit soil borne fungal growth and non-toxic to silkworm without affecting larval growth and cocoon weight under the laboratory conditions tested.

研究了荧光假单胞菌（Pseudomonas fluorescens，Pf）对土壤传播的真菌病原菌的拮抗作用以及Pf对家蚕（Bombyx mori L.）的生殖毒性。的拮抗性荧光假单胞菌菌株即，荧光假单胞菌5（PF 5），荧光假单胞菌18（PF 18）和荧光假单胞菌25（PF 25）通过对土壤传播的真菌脉冲病原体即体外双重培养方法进行评价。 ，镰刀菌属。，链格孢菌，菜豆大单胞菌和茄霉。多菌灵50％WP作为阳性标准，而无拮抗剂（Pf）的真菌病原体作为对照组。在所有治疗组和对照组中均记录了菌丝生长。通过单向方差分析对不同菌株的结果进行统计学比较。以多菌灵> Pf 5> Pf 25> Pf 18的顺序记录菌丝体对对照的真菌病原体生长的抑制作用。通过叶浸法评价对家蚕的毒性。测试的治疗是Pf 5，Pf 18，Pf 25 @ 10 ⁸ cfu / mL和乐果30％EC用作阳性标准品。将叶子浸入去离子水中作为对照。与对照组相比，在死亡率，幼体持续时间和茧重数据上的治疗没有明显差异。饲料消耗也与对照组相当。因此，在测试的实验室条件下，发现选定的分离株可抑制土壤传播的真菌生长，并且对蚕无毒，而不会影响幼虫的生长和茧重。