ABSTRACT

Introduction: Molecular technology has played an important role in arboviruses diagnostics. PCR-based methods stand out in terms of sensitivity, specificity, cost, robustness, and accessibility, and especially the isothermal amplification (IA) method is ideal for field-adaptable diagnostics in resource-limited settings (RLS).

Areas covered: In this review, we provide an overview of the various molecular methods for West Nile, Zika, Dengue and Chikungunya. We summarize literature works reporting the assessment and use of in house and commercial assays. We describe limitations and challenges in the usage of methods and opportunities for novel approaches such as NNext-GenerationSequencing (NGS).

Expert opinion: The rapidity and accuracy of differential diagnosis is essential for a successful clinical management, particularly in co-circulation area of arboviruses. Several commercial diagnostic molecular assays are available, but many are not affordable by RLS and not usable as Point-of-care/Point-of-need (POC/PON) such as RReal-TimeRT-PCR, Array-based methods and NGS. In contrast, the IA-based system fits better for POC/PON but it is still not ideal for the multiplexing detection system. Improvement in the characterization and validation of current molecular assays is needed to optimize their translation to the point of care.

摘要

简介：分子技术在虫媒病毒诊断中发挥了重要作用。基于 PCR 的方法在灵敏度、特异性、成本、稳健性和可及性方面脱颖而出，尤其是等温扩增 (IA) 方法是资源有限环境 (RLS) 中现场适应性诊断的理想选择。

涵盖的领域：在这篇评论中，我们概述了西尼罗河、寨卡、登革热和基孔肯雅热的各种分子方法。我们总结了报告内部和商业化验评估和使用的文献工作。我们描述了使用新方法（例如 NNext-GenerationSequencing (NGS)）的方法和机会的局限性和挑战。

专家意见：鉴别诊断的快速性和准确性对于临床管理的成功至关重要，特别是在虫媒病毒的共循环领域。有几种商业诊断分子检测方法可供使用，但 RLS 负担不起许多商业诊断分子检测方法，也不能用作护理点/需求点 (POC/PON)，例如 RReal-TimeRT-PCR、基于阵列的方法和 NGS。相比之下，基于 IA 的系统更适合 POC/PON，但仍不适合多路复用检测系统。需要改进当前分子检测的表征和验证，以优化它们到护理点的转化。