Background: The genomic copy number of LINC01061 is amplified in papillary thyroid cancer. However, its role in gastric cancer is not clear. Materials and Methods: Tissues and serum of GC patients were collected to detect the expression of LINC01061 by quantitative real-time polymerase chain reaction (qRT-PCR). ShRNA were applied to knock down the expression of LINC01061. Growth curves and colony formation experiments were applied to evaluate cell growth. Cell migration was assessed by transwell migration experiments. Cell cycle and apoptosis were analyzed by flow cytometry. Epithelial-mesenchymal transition (EMT) was examined by qRT-PCR and Western blot. Results: The expression of LINC01061 was upregulated in tissues and serum of GC patients and it was associated with the clinicopathological features and survival time. Functional study indicated that cell growth and migration were suppressed after LINC01061 knockdown. Cell cycle arrest and increased apoptosis occurred when LINC01061 expression was inhibited. EMT was also impaired combined with a decrease in β-catenin expression after LINC01061 knockdown. Conclusions: Our data indicate that LINC01061 is a novel biomarker for diagnosis and prognosis of GC. LINC01061 promoted progression of GC through cell cycle regulation and EMT.
Oncol Res Treat

中文翻译：

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增强 LINC01061 水平作为胃癌血清生物标志物和促进恶性转化

背景： LINC01061 的基因组拷贝数在甲状腺乳头状癌中扩增。然而，其在胃癌中的作用尚不清楚。材料与方法：收集GC患者的组织和血清，采用定量实时聚合酶链反应（qRT-PCR）检测LINC01061的表达。应用 ShRNA 来抑制 LINC01061 的表达。应用生长曲线和集落形成实验来评估细胞生长。通过跨孔迁移实验评估细胞迁移。通过流式细胞术分析细胞周期和细胞凋亡。通过 qRT-PCR 和蛋白质印迹检查上皮间质转化 (EMT)。结果：LINC01061在胃癌患者的组织和血清中表达上调，并与临床病理特征和生存时间有关。功能研究表明，LINC01061 敲低后细胞生长和迁移受到抑制。当 LINC01061 表达被抑制时，发生细胞周期停滞和细胞凋亡增加。敲除 LINC01061 后，EMT 也受损，并伴有 β-catenin 表达的降低。结论：我们的数据表明，LINC01061 是一种用于 GC 诊断和预后的新型生物标志物。LINC01061 通过细胞周期调节和 EMT 促进 GC 的进展。
肿瘤资源治疗