Introduction: The pathogenesis of chronic rhinosinusitis (CRS) with nasal polyps (CRSwNPs) is not yet completely understood. Based on current knowledge, the infiltration of mast cells and eosinophils in nasal polyps (NPs) plays an important role. This study aimed to investigate the interplay of asthma and allergy etiopathology in CRSwNPs patients by specifically studying tissue mast cells and eosinophils and the pro-inflammatory marker CD48. Methods: Immunohistochemistry was used to assess eosinophils, mast cells, and CD48 expressing eosinophils infiltrating NPs, and flow cytometry was used to assess surface receptors expression on eosinophils from digested NPs. Results: Immunohistochemical analyses showed that mast cell infiltration in NPs is higher in allergic patients in comparison to nonallergic patients; eosinophils infiltration in asthmatic NPs was significantly elevated in comparison to the nonasthmatic NPs, and membrane CD48 (mCD48) expression on eosinophils infiltrating nonallergic asthmatic NPs was highly elevated in comparison to the other subgroups. Similarly, mCD48 and its high-affinity ligand m2B4’s expression on eosinophils from enzymatically digested NPs were significantly higher in nonallergic asthmatics in comparison to allergic asthmatics. Conclusions: Eosinophil infiltration in NPs for asthmatic patients, and mast cell infiltration for allergic patients, may be used as reliable biomarkers for endotyping CRSwNPs. In addition, CD48 in asthmatic patients who developed CRSwNPs could be regarded as a potential target for treatment.
Int Arch Allergy Immunol

中文翻译：

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CD48在慢性鼻窦炎患者鼻息肉中嗜酸性粒细胞的表达

简介：慢性鼻窦炎（CRS）伴鼻息肉（CRSwNPs）的发病机制尚未完全了解。根据目前的知识，鼻息肉 (NPs) 中肥大细胞和嗜酸性粒细胞的浸润起着重要作用。本研究旨在通过专门研究组织肥大细胞和嗜酸性粒细胞以及促炎标志物 CD48，研究 CRSwNPs 患者中哮喘和过敏病理学的相互作用。方法：免疫组织化学用于评估浸润 NP 的嗜酸性粒细胞、肥大细胞和表达 CD48 的嗜酸性粒细胞，流式细胞术用于评估来自消化的 NP 的嗜酸性粒细胞表面受体的表达。结果：免疫组织化学分析表明，与非过敏患者相比，过敏患者的 NPs 中的肥大细胞浸润更高；与非哮喘 NP 相比，哮喘 NP 中的嗜酸性粒细胞浸润显着升高，并且与其他亚组相比，浸润非过敏性哮喘 NP 的嗜酸性粒细胞上的膜 CD48 (mCD48) 表达高度升高。类似地，与过敏性哮喘患者相比，mCD48 及其高亲和力配体 m2B4 在来自酶消化 NP 的嗜酸性粒细胞上的表达在非过敏性哮喘患者中显着更高。结论：哮喘患者的 NPs 中的嗜酸性粒细胞浸润和过敏患者的肥大细胞浸润可用作内分型 CRSwNPs 的可靠生物标志物。此外，发生 CRSwNPs 的哮喘患者的 CD48 可被视为潜在的治疗靶点。
Int Arch 过敏免疫